TY - JOUR
T1 - Arachldonic acid metabolic pathways in the rabbit pericardium
AU - Honda, Atsushi
AU - Morrison, Aubrey R.
AU - McCluskey, Edward R.
AU - Needleman, Philip
N1 - Funding Information:
This work was supported by NIH grants SCOR-HL17646 (P.N.), training grant HL-07275 (E.R.M.) and AM30542-02 (A.R.M.) and Fogarty International Fellowship No. F05 TWOA3280-01 (A.H.). We thank W.J. Elliott, M.D., Ph.D., for his helpful suggestions and advice. A.R.M. is an Established Investigator of AHA.
PY - 1984/7/26
Y1 - 1984/7/26
N2 - Minced rabbit pericardium actively converts [1-14C]arachidonic acid into the known prostaglandins (6-[1-14C] ketoprostagl and in F1α, [1-14C] prostagl and in E2 and [1-14C] prostagl and in F2α) and into several unidentified metabolites. The major metabolite was separated by C18 reverse-phase high-pressure liquid chromatography (HPLC) and identified by gas chromatography-mass spectrometry (GC-MS) to be 6,15-[1-14C]diketo-13,14-dihydroprostagl and in F1α. The other nonpolar metabolites were 15-[1-14C]hydroxy-5,8,11,13-eicosatetraenoic acid (15-HETE), 11-[1-14C]hydroxy-5,8,12,14-eicosatetraenoicacid (11-HETE) and 12-(1-14C]hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE). Arachidonic acid metabolites actively produced by the pericardium could influence the tone of surface blood vessels on the myocardium:.
AB - Minced rabbit pericardium actively converts [1-14C]arachidonic acid into the known prostaglandins (6-[1-14C] ketoprostagl and in F1α, [1-14C] prostagl and in E2 and [1-14C] prostagl and in F2α) and into several unidentified metabolites. The major metabolite was separated by C18 reverse-phase high-pressure liquid chromatography (HPLC) and identified by gas chromatography-mass spectrometry (GC-MS) to be 6,15-[1-14C]diketo-13,14-dihydroprostagl and in F1α. The other nonpolar metabolites were 15-[1-14C]hydroxy-5,8,11,13-eicosatetraenoic acid (15-HETE), 11-[1-14C]hydroxy-5,8,12,14-eicosatetraenoicacid (11-HETE) and 12-(1-14C]hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE). Arachidonic acid metabolites actively produced by the pericardium could influence the tone of surface blood vessels on the myocardium:.
KW - (Rabbit pericardium)
KW - Arachidonate metabolism
KW - Eicosanoid
KW - Prostacyclin catabolism
KW - Prostaglandin
UR - http://www.scopus.com/inward/record.url?scp=0021264569&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(84)90006-7
DO - 10.1016/0005-2760(84)90006-7
M3 - Article
C2 - 6430346
AN - SCOPUS:0021264569
SN - 0005-2760
VL - 794
SP - 403
EP - 410
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 3
ER -