Arachidonic acid metabolic pathway of the rabbit placenta

Michele H. Bloch; Laura L. McLaughlin; J. Crowley; P. Needleman; A. R. Morrison

doi:10.1016/0090-6980(85)90202-3

Arachidonic acid metabolic pathway of the rabbit placenta

Michele H. Bloch
, Laura L. McLaughlin
, J. Crowley
, P. Needleman
, A. R. Morrison

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Placenta microsomes prepared from animals late in gestation (29 days) efficiently metabolize arachidonic acid into PGE₂, PGF_2α, PGD₂, PGD₂, TxA₂ and little or no prostacyclin. In contrast to the late gestation placenta, the early (17 day) placental microsomes synthesize primarily PGE₂. The cytosolic (100, 000 × g supernatant) fraction from early or late gestation placentae converted arachidonic acid, with a calcium dependent enzyme, into non-polar metabolites whose synthesis was inhibited by ETYA but not indomethacin. These metabolites were purified by HPLC and GC-MS analysis indicated the presence of 12-hydroxy-, 15-hydroxy-, and 11-hydroxy-eicosatetraenoic acid. The mitochondrial (8,000 × g pellet) produced PGE₂; PGF_2α; 12-. 11-, 15-HETE; the C-17 fragment HHT; and the unusual cyclooxygenase metabolite 15-keto-PGE₂. These biologically active metabolites may play a vital role in the reproductive function of the placenta.

Original language	English
Pages (from-to)	203-216
Number of pages	14
Journal	Prostaglandins
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0090-6980(85)90202-3
State	Published - Feb 1985

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@article{a829a329c6d7402b9a42963cd399974a,

title = "Arachidonic acid metabolic pathway of the rabbit placenta",

abstract = "Placenta microsomes prepared from animals late in gestation (29 days) efficiently metabolize arachidonic acid into PGE2, PGF2α, PGD2, PGD2, TxA2 and little or no prostacyclin. In contrast to the late gestation placenta, the early (17 day) placental microsomes synthesize primarily PGE2. The cytosolic (100, 000 {\texttimes} g supernatant) fraction from early or late gestation placentae converted arachidonic acid, with a calcium dependent enzyme, into non-polar metabolites whose synthesis was inhibited by ETYA but not indomethacin. These metabolites were purified by HPLC and GC-MS analysis indicated the presence of 12-hydroxy-, 15-hydroxy-, and 11-hydroxy-eicosatetraenoic acid. The mitochondrial (8,000 {\texttimes} g pellet) produced PGE2; PGF2α; 12-. 11-, 15-HETE; the C-17 fragment HHT; and the unusual cyclooxygenase metabolite 15-keto-PGE2. These biologically active metabolites may play a vital role in the reproductive function of the placenta.",

author = "Bloch, \{Michele H.\} and McLaughlin, \{Laura L.\} and J. Crowley and P. Needleman and Morrison, \{A. R.\}",

note = "Funding Information: This work is supported by NIH part HL1439 and HL20787 (PN) and 30562-02 (ARM). Dr. \textasciitilde{}orrison is an established investigator of the American Heart Association.",

year = "1985",

month = feb,

doi = "10.1016/0090-6980(85)90202-3",

language = "English",

volume = "29",

pages = "203--216",

journal = "Prostaglandins",

issn = "0090-6980",

number = "2",

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TY - JOUR

T1 - Arachidonic acid metabolic pathway of the rabbit placenta

AU - Bloch, Michele H.

AU - McLaughlin, Laura L.

AU - Crowley, J.

AU - Needleman, P.

AU - Morrison, A. R.

N1 - Funding Information: This work is supported by NIH part HL1439 and HL20787 (PN) and 30562-02 (ARM). Dr. ~orrison is an established investigator of the American Heart Association.

PY - 1985/2

Y1 - 1985/2

N2 - Placenta microsomes prepared from animals late in gestation (29 days) efficiently metabolize arachidonic acid into PGE2, PGF2α, PGD2, PGD2, TxA2 and little or no prostacyclin. In contrast to the late gestation placenta, the early (17 day) placental microsomes synthesize primarily PGE2. The cytosolic (100, 000 × g supernatant) fraction from early or late gestation placentae converted arachidonic acid, with a calcium dependent enzyme, into non-polar metabolites whose synthesis was inhibited by ETYA but not indomethacin. These metabolites were purified by HPLC and GC-MS analysis indicated the presence of 12-hydroxy-, 15-hydroxy-, and 11-hydroxy-eicosatetraenoic acid. The mitochondrial (8,000 × g pellet) produced PGE2; PGF2α; 12-. 11-, 15-HETE; the C-17 fragment HHT; and the unusual cyclooxygenase metabolite 15-keto-PGE2. These biologically active metabolites may play a vital role in the reproductive function of the placenta.

AB - Placenta microsomes prepared from animals late in gestation (29 days) efficiently metabolize arachidonic acid into PGE2, PGF2α, PGD2, PGD2, TxA2 and little or no prostacyclin. In contrast to the late gestation placenta, the early (17 day) placental microsomes synthesize primarily PGE2. The cytosolic (100, 000 × g supernatant) fraction from early or late gestation placentae converted arachidonic acid, with a calcium dependent enzyme, into non-polar metabolites whose synthesis was inhibited by ETYA but not indomethacin. These metabolites were purified by HPLC and GC-MS analysis indicated the presence of 12-hydroxy-, 15-hydroxy-, and 11-hydroxy-eicosatetraenoic acid. The mitochondrial (8,000 × g pellet) produced PGE2; PGF2α; 12-. 11-, 15-HETE; the C-17 fragment HHT; and the unusual cyclooxygenase metabolite 15-keto-PGE2. These biologically active metabolites may play a vital role in the reproductive function of the placenta.

UR - https://www.scopus.com/pages/publications/0021942246

U2 - 10.1016/0090-6980(85)90202-3

DO - 10.1016/0090-6980(85)90202-3

M3 - Article

C2 - 3920728

AN - SCOPUS:0021942246

SN - 0090-6980

VL - 29

SP - 203

EP - 216

JO - Prostaglandins

JF - Prostaglandins

IS - 2

ER -

Arachidonic acid metabolic pathway of the rabbit placenta

Abstract

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