TY - JOUR
T1 - Arachidonic acid induces an increase in the cytosolic calcium concentration in single pancreatic islet beta cells
AU - Ramanadham, Sasanka
AU - Gross, Richard
AU - Turk, John
N1 - Funding Information:
ACKNOWLEDGMENTS. We acknowledge support by NIH grants HL-DK-34388 and DK-01553 and by the Monsanto Co. and the superb assistance of Alan Bohrer, Mary Mueller, Kelly Kruszka and Lori
PY - 1992/4/30
Y1 - 1992/4/30
N2 - The insulin secretagogue D-glucose induces both accumulation of nonesterified arachidonic acid (35 μM) in pancreatic islets and a rise in beta cell cytosolic [Ca++]i. Arachidonate amplifies both voltage-dependent Ca++ entry in secretory cells and depolarization-induced insulin secretion. Here, arachidonate induced a biphasic rise in [Ca++]i of Fura-2AM loaded beta cells which increased with arachidonate concentration (5-30 μM), was reversed upon washout, and was unaffected by the arachidonate oxygenase inhibitor BW755C. The sustained phase of the rise was abolished by removal of extracellular Ca++ and amplified by depolarization with KCl. The accumulation of nonesterified arachidonate in islets stimulated by D-glucose may therefore promote the D-glucose-induced rise in beta cell [Ca++]i.
AB - The insulin secretagogue D-glucose induces both accumulation of nonesterified arachidonic acid (35 μM) in pancreatic islets and a rise in beta cell cytosolic [Ca++]i. Arachidonate amplifies both voltage-dependent Ca++ entry in secretory cells and depolarization-induced insulin secretion. Here, arachidonate induced a biphasic rise in [Ca++]i of Fura-2AM loaded beta cells which increased with arachidonate concentration (5-30 μM), was reversed upon washout, and was unaffected by the arachidonate oxygenase inhibitor BW755C. The sustained phase of the rise was abolished by removal of extracellular Ca++ and amplified by depolarization with KCl. The accumulation of nonesterified arachidonate in islets stimulated by D-glucose may therefore promote the D-glucose-induced rise in beta cell [Ca++]i.
UR - http://www.scopus.com/inward/record.url?scp=0026720003&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(92)90638-2
DO - 10.1016/0006-291X(92)90638-2
M3 - Article
C2 - 1575739
AN - SCOPUS:0026720003
SN - 0006-291X
VL - 184
SP - 647
EP - 653
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -