Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities

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Abstract

Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized.

Original languageEnglish
Article number158
JournalMicrobiome
Volume7
Issue number1
DOIs
StatePublished - Dec 12 2019

Keywords

  • Body composition
  • Energy expenditure
  • Gnotobiotic mouse husbandry
  • Host phenotyping
  • Human donor gut bacterial culture collections
  • Human gut microbiota
  • Obesity
  • Open-circuit indirect calorimetry

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