TY - JOUR
T1 - Applying genomics to the avian inner ear
T2 - Development of subtractive cDNA resources for exploring sensory function and hair cell regeneration
AU - Hawkins, R. David
AU - Helms, Cynthia A.
AU - Winston, Julia B.
AU - Warchol, Mark E.
AU - Lovett, Michael
N1 - Funding Information:
This work was supported by a grant from the National Organization of Hearing Research Foundation.
PY - 2006/6
Y1 - 2006/6
N2 - We applied a micro-cDNA-based subtraction method to identify genes expressed in the regenerating sensory epithelia (SE) of the chicken inner ear. Sensory hair cells in the avian utricle SE are in a constant state of turnover, where dying hair cells are replaced by new ones derived from supporting cells. In contrast, hair cells in the cochlea remain quiescent unless damaged. We used this difference to enrich for utricle-specific genes, using reiterative cDNA subtraction and demonstrate enrichment for utricle-specific sequences. A total of 1710 cDNA sequence reads revealed the presence of many cDNAs encoding known structural components of the SE (for example, Harmonin and beta-tectorin), proteins involved in cellular proliferation, such as P311, HIPK2, and SPALT1, among many others of unknown function. These libraries are the first of their kind and should prove useful for the discovery of candidate genes for hearing disorders, regenerative and apoptotic pathways, and novel chicken ESTs.
AB - We applied a micro-cDNA-based subtraction method to identify genes expressed in the regenerating sensory epithelia (SE) of the chicken inner ear. Sensory hair cells in the avian utricle SE are in a constant state of turnover, where dying hair cells are replaced by new ones derived from supporting cells. In contrast, hair cells in the cochlea remain quiescent unless damaged. We used this difference to enrich for utricle-specific genes, using reiterative cDNA subtraction and demonstrate enrichment for utricle-specific sequences. A total of 1710 cDNA sequence reads revealed the presence of many cDNAs encoding known structural components of the SE (for example, Harmonin and beta-tectorin), proteins involved in cellular proliferation, such as P311, HIPK2, and SPALT1, among many others of unknown function. These libraries are the first of their kind and should prove useful for the discovery of candidate genes for hearing disorders, regenerative and apoptotic pathways, and novel chicken ESTs.
KW - Hair cells
KW - Library subtraction
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=33646829799&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2005.12.014
DO - 10.1016/j.ygeno.2005.12.014
M3 - Article
C2 - 16516437
AN - SCOPUS:33646829799
SN - 0888-7543
VL - 87
SP - 801
EP - 808
JO - Genomics
JF - Genomics
IS - 6
ER -