TY - JOUR
T1 - Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation
AU - Bhowmik, Salil Kumar
AU - Putluri, Vasanta
AU - Kommagani, Ramakrishna
AU - Konde, Sai Aparna
AU - Lydon, John P.
AU - Sreekumar, Arun
AU - Putluri, Nagireddy
PY - 2013/12
Y1 - 2013/12
N2 - Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C-labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.
AB - Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C-labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.
KW - metabolic relative flux
KW - single reaction monitoring
KW - tandem mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84890478412&partnerID=8YFLogxK
U2 - 10.1002/jms.3292
DO - 10.1002/jms.3292
M3 - Article
C2 - 24338880
AN - SCOPUS:84890478412
SN - 1076-5174
VL - 48
SP - 1270
EP - 1275
JO - Journal of Mass Spectrometry
JF - Journal of Mass Spectrometry
IS - 12
ER -