Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation

Salil Kumar Bhowmik, Vasanta Putluri, Ramakrishna Kommagani, Sai Aparna Konde, John P. Lydon, Arun Sreekumar, Nagireddy Putluri

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C-labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.

Original languageEnglish
Pages (from-to)1270-1275
Number of pages6
JournalJournal of Mass Spectrometry
Volume48
Issue number12
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

Keywords

  • metabolic relative flux
  • single reaction monitoring
  • tandem mass spectrometry

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