@inbook{dd326a240bf04bfbb461d35714bdf691,
title = "Application of confocal single-molecule FRET to intrinsically disordered proteins",
abstract = "Intrinsically disordered proteins (IDPs) are characterized by a large degree of conformational heterogeneity. In such cases, classical experimental methods often yield only mean values, averaged over the entire ensemble of molecules. The microscopic distributions of conformations, trajectories, or sequences of events often remain unknown, and with them the underlying molecular mechanisms. Signal averaging can be avoided by observing individual molecules. A particularly versatile method is highly sensitive fluorescence detection. In combination with F{\"o}rster resonance energy transfer (FRET), distances and conformational dynamics can be investigated in single molecules. This chapter introduces the practical aspects of applying confocal single-molecule FRET experiments to the study of IDPs.",
keywords = "Confocal detection, Correlation spectroscopy, Diffusion, FCS, FRET, Fluorescence spectroscopy, F{\"o}rster resonance energy transfer, Intrinsically disordered proteins, Photon statistics, Single-molecule detection",
author = "Benjamin Schuler and Sonja M{\"u}ller-Sp{\"a}th and Andrea Soranno and Daniel Nettels",
year = "2012",
doi = "10.1007/978-1-4614-3704-8_2",
language = "English",
isbn = "9781461437031",
series = "Methods in Molecular Biology",
pages = "21--45",
editor = "Vladimir Uversky and Vladimir Uversky and Keith Dunker",
booktitle = "Intrinsically Disordered Protein Analysis",
}