TY - JOUR
T1 - Ap1-like cis elements in the 5′-regulatory region of the human apolipoprotein A-I gene
AU - Lapikov, I. A.
AU - Mogilenko, D. A.
AU - Dizhe, E. B.
AU - Ignatovich, I. A.
AU - Orlov, S. V.
AU - Perevozchikov, A. P.
N1 - Funding Information:
This work was supported by the Russian Foundation for Basic Research (project no. 07-04-1-110).
PY - 2008/4
Y1 - 2008/4
N2 - Ap1-like cis elements, interacting with transcription factors of the Ap1 and CREB/ATF families, were identified in the 5′-regulatory region of the human apolipoprotein A-I gene (5′-apoA-I). The elements are beyond the hepatic enhancer (-220...-110) and region -595...-192, responsible for efficient transcription in Caco2 cells. One of the elements (5′-TGAGGTCT-3′, Cre/jun2/apo) occurs in 5′-apoA-I in two copies, distal (-2176...-2165) and proximal (99...106). The electrophoretic mobility shift assay was used to characterize this and two other 5′-apoA-I Ap1-like elements: 5′-TGACTCT-3′ (-1798...-1791, PF1) and 5′-TGACATCA-3′ (-1171...-1163, Cre/jun1). Experiments with specific antibodies identified ATF2 as a component of the complexes formed by HepG2 nuclear proteins with Cre/jun2/apo and Cre/jun1. Several 5′-apoA-I deletion derivatives were examined in HepG2 hepatoma cells (producing ApoA-I), Hutu80 duodenal adenocarcinoma cells (lacking ApoA-I production), and SK-N-SH neuroblastoma cells (carrying defective apoA-I) by the luciferase reporter assay. Combined with cell cotransfection with c-jun and mekk1 expression vectors, the assays implicated the Ap1-like elements in the tissue-specific regulation of apoA-I expression, the proximal Cre/jun2/apo element playing the major role.
AB - Ap1-like cis elements, interacting with transcription factors of the Ap1 and CREB/ATF families, were identified in the 5′-regulatory region of the human apolipoprotein A-I gene (5′-apoA-I). The elements are beyond the hepatic enhancer (-220...-110) and region -595...-192, responsible for efficient transcription in Caco2 cells. One of the elements (5′-TGAGGTCT-3′, Cre/jun2/apo) occurs in 5′-apoA-I in two copies, distal (-2176...-2165) and proximal (99...106). The electrophoretic mobility shift assay was used to characterize this and two other 5′-apoA-I Ap1-like elements: 5′-TGACTCT-3′ (-1798...-1791, PF1) and 5′-TGACATCA-3′ (-1171...-1163, Cre/jun1). Experiments with specific antibodies identified ATF2 as a component of the complexes formed by HepG2 nuclear proteins with Cre/jun2/apo and Cre/jun1. Several 5′-apoA-I deletion derivatives were examined in HepG2 hepatoma cells (producing ApoA-I), Hutu80 duodenal adenocarcinoma cells (lacking ApoA-I production), and SK-N-SH neuroblastoma cells (carrying defective apoA-I) by the luciferase reporter assay. Combined with cell cotransfection with c-jun and mekk1 expression vectors, the assays implicated the Ap1-like elements in the tissue-specific regulation of apoA-I expression, the proximal Cre/jun2/apo element playing the major role.
KW - 5′-regulatory region of the human apolipoprotein A-I gene
KW - ATF2
KW - Ap1
KW - Ap1-like cis elements
KW - HepG2 hepatoma cell line
KW - Human cell lines
KW - Hutu80 duodenal carcinoma cell line
KW - Mekk1 protein kinase
KW - SK-N-SH neuroblastoma cell line
KW - Transcription factors
UR - http://www.scopus.com/inward/record.url?scp=44349093844&partnerID=8YFLogxK
U2 - 10.1134/S002689330802012X
DO - 10.1134/S002689330802012X
M3 - Article
AN - SCOPUS:44349093844
SN - 0026-8933
VL - 42
SP - 261
EP - 269
JO - Molecular Biology
JF - Molecular Biology
IS - 2
ER -