AP-2-containing clathrin coats assemble on mature lysosomes

Linton M. Traub, Sergei I. Bannykh, Jill E. Rodel, Meir Aridor, William E. Balch, Stuart Kornfeld

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Abstract

Coat proteins appear to play a general role in intracellular protein trafficking by coordinating a membrane budding event with cargo selection. Here we show that the AP-2 adaptor, a clathrin-associated coat-protein complex that nucleates clathrin-coated vesicle formation at the cell surface, can also initiate the assembly of normal polyhedral clathrin coats on dense lysosomes under physiological conditions in vitro. Clathrin coal formation on lysosomes is temperature dependent, displays an absolute requirement for ATP, and occurs in both semi-intact cells and on purified lysosomes, suggesting that clathrin-coated vesicles might regulate retrograde membrane traffic out of the lysosomal compartment.

Original languageEnglish
Pages (from-to)1801-1814
Number of pages14
JournalJournal of Cell Biology
Volume135
Issue number6 II
DOIs
StatePublished - Dec 1 1996
Externally publishedYes

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    Traub, L. M., Bannykh, S. I., Rodel, J. E., Aridor, M., Balch, W. E., & Kornfeld, S. (1996). AP-2-containing clathrin coats assemble on mature lysosomes. Journal of Cell Biology, 135(6 II), 1801-1814. https://doi.org/10.1083/jcb.135.6.1801