TY - JOUR
T1 - Anti—tumor necrosis factor therapy abrogates autoimmune demyelination
AU - Selmaj, K.
AU - Raine, C. S.
AU - Cross, A. H.
PY - 1991/11
Y1 - 1991/11
N2 - To define a role for the cytokine tumor necrosis factor (TNF) in immune‐mediated demyelination, the effect of anti‐TNF antibody was investigated with a form of experimental autoimmune encephalomyelitis (EAE) in SJL/J mice induced by the adoptive transfer of myelin basic protein‐(MBP)‐sensitized T lymphocytes, an animal model of the human disease multiple sclerosis (MS). In three separate experiments, no mouse sensitized for EAE and then treated with anti‐TNF by intraperitoneal injection developed signs of central nervous system (CNS) disease. Examination of CNS tissue from anti‐TNF‐treated animals showed no pathological changes. CNS tissue from control animals demonstrated extensive inflammatory cell infiltration and demyelination. To test whether anti‐TNF therapy was inhibitory to encephalitogenic cells, preincubation of MBP‐sensitized T lymphocytes with anti‐TNF in vitro prior to injection into recipient mice was performed, and resulted in no diminution of their ability to transfer EAE. In addition, spleen cells from anti‐TNF‐ treated mice were capable of serial transfer of EAE, similar to spleen cells from control animals. However, spleen cells from anti‐TNF‐treated mice did not produce TNF on stimulation with MBP or concanavalin A. This study showed that anti‐TNF antibody can inhibit effectively the development of EAE by interfering with the effector, rather than the induction, phase of the disease. Anticytokine therapy may have important applications in the development of new therapeutic strategies for MS.
AB - To define a role for the cytokine tumor necrosis factor (TNF) in immune‐mediated demyelination, the effect of anti‐TNF antibody was investigated with a form of experimental autoimmune encephalomyelitis (EAE) in SJL/J mice induced by the adoptive transfer of myelin basic protein‐(MBP)‐sensitized T lymphocytes, an animal model of the human disease multiple sclerosis (MS). In three separate experiments, no mouse sensitized for EAE and then treated with anti‐TNF by intraperitoneal injection developed signs of central nervous system (CNS) disease. Examination of CNS tissue from anti‐TNF‐treated animals showed no pathological changes. CNS tissue from control animals demonstrated extensive inflammatory cell infiltration and demyelination. To test whether anti‐TNF therapy was inhibitory to encephalitogenic cells, preincubation of MBP‐sensitized T lymphocytes with anti‐TNF in vitro prior to injection into recipient mice was performed, and resulted in no diminution of their ability to transfer EAE. In addition, spleen cells from anti‐TNF‐ treated mice were capable of serial transfer of EAE, similar to spleen cells from control animals. However, spleen cells from anti‐TNF‐treated mice did not produce TNF on stimulation with MBP or concanavalin A. This study showed that anti‐TNF antibody can inhibit effectively the development of EAE by interfering with the effector, rather than the induction, phase of the disease. Anticytokine therapy may have important applications in the development of new therapeutic strategies for MS.
UR - http://www.scopus.com/inward/record.url?scp=0026094706&partnerID=8YFLogxK
U2 - 10.1002/ana.410300510
DO - 10.1002/ana.410300510
M3 - Article
C2 - 1722388
AN - SCOPUS:0026094706
SN - 0364-5134
VL - 30
SP - 694
EP - 700
JO - Annals of neurology
JF - Annals of neurology
IS - 5
ER -