Antigenicity of Elastin: Characterization of Major Antigenic Determinants on Purified Insoluble Elastin

Robert P. Mecham; Gary Lange

doi:10.1021/bi00533a013

Antigenicity of Elastin: Characterization of Major Antigenic Determinants on Purified Insoluble Elastin

Robert P. Mecham, Gary Lange

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

This investigation reports a systematic comparison of antibody responses to well-defined fragments of mature, insoluble elastin in order to identify antigenic regions of the molecule. Antiserum to insoluble elastin was used to screen by radioimmunoassay the serologic activity of elastin peptides purified from a thermolysin digestion of insoluble elastin. Fractions with positive antigenicity were characterized by amino acid analysis and protein sequence analysis. Our results indicate that antigenic determinants on elastin can be classified functionally into two categories: major determinants related to the cross-linking domain which show cross-reactivity between species and limited, species-specific determinants in non-cross-linked regions of the molecule.

Original language	English
Pages (from-to)	669-673
Number of pages	5
Journal	Biochemistry
Volume	21
Issue number	4
DOIs	https://doi.org/10.1021/bi00533a013
State	Published - Jan 1 1982

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title = "Antigenicity of Elastin: Characterization of Major Antigenic Determinants on Purified Insoluble Elastin",

abstract = "This investigation reports a systematic comparison of antibody responses to well-defined fragments of mature, insoluble elastin in order to identify antigenic regions of the molecule. Antiserum to insoluble elastin was used to screen by radioimmunoassay the serologic activity of elastin peptides purified from a thermolysin digestion of insoluble elastin. Fractions with positive antigenicity were characterized by amino acid analysis and protein sequence analysis. Our results indicate that antigenic determinants on elastin can be classified functionally into two categories: major determinants related to the cross-linking domain which show cross-reactivity between species and limited, species-specific determinants in non-cross-linked regions of the molecule.",

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AB - This investigation reports a systematic comparison of antibody responses to well-defined fragments of mature, insoluble elastin in order to identify antigenic regions of the molecule. Antiserum to insoluble elastin was used to screen by radioimmunoassay the serologic activity of elastin peptides purified from a thermolysin digestion of insoluble elastin. Fractions with positive antigenicity were characterized by amino acid analysis and protein sequence analysis. Our results indicate that antigenic determinants on elastin can be classified functionally into two categories: major determinants related to the cross-linking domain which show cross-reactivity between species and limited, species-specific determinants in non-cross-linked regions of the molecule.

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