Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67

Shan Rong Shi; Benjaporn Chaiwun; Lillian Young; Ashraf Imam; Richard J. Cote; Clive R. Taylor

doi:10.3109/10520299409106289

Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67

Shan Rong Shi, Benjaporn Chaiwun, Lillian Young, Ashraf Imam, Richard J. Cote, Clive R. Taylor

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Abstract

A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

Original language	English
Pages (from-to)	213-215
Number of pages	3
Journal	Biotechnic and Histochemistry
Volume	69
Issue number	4
DOIs	https://doi.org/10.3109/10520299409106289
State	Published - 1994

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title = "Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67",

abstract = "A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.",

author = "Shi, {Shan Rong} and Benjaporn Chaiwun and Lillian Young and Ashraf Imam and Cote, {Richard J.} and Taylor, {Clive R.}",

note = "Funding Information: This work was supported by University Pathology Associates of the University of Southern California.",

year = "1994",

doi = "10.3109/10520299409106289",

language = "English",

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journal = "Biotechnic and Histochemistry",

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T1 - Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67

AU - Shi, Shan Rong

AU - Chaiwun, Benjaporn

AU - Young, Lillian

AU - Imam, Ashraf

AU - Cote, Richard J.

AU - Taylor, Clive R.

N1 - Funding Information: This work was supported by University Pathology Associates of the University of Southern California.

PY - 1994

Y1 - 1994

N2 - A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

AB - A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

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U2 - 10.3109/10520299409106289

DO - 10.3109/10520299409106289

M3 - Article

C2 - 7918836

AN - SCOPUS:0028364960

SN - 1052-0295

VL - 69

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JO - Biotechnic and Histochemistry

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ER -

Antigen retrieval using ph 3.5 glycine-HCI buffer or urea solution for immunohistochemical localization of ki-67

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