Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: Standardization and development

Shan Rong Shi; Richard J. Cote; Clive R. Taylor

doi:10.1016/S0385-8146(98)00042-X

Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: Standardization and development

Shan Rong Shi, Richard J. Cote, Clive R. Taylor

Research output: Contribution to journal › Review article › peer-review

23 Scopus citations

Abstract

The use of immunohistochemistry (IHC) in routinely processed celloidin- embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.

Original language	English
Pages (from-to)	425-443
Number of pages	19
Journal	Auris Nasus Larynx
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/S0385-8146(98)00042-X
State	Published - Dec 1998

Keywords

Antigen retrieval
Celloidin-embedding tissue
Human temporal bone
Immunohistochemistry
Microwave
Otopathology

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10.1016/S0385-8146(98)00042-X

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title = "Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: Standardization and development",

abstract = "The use of immunohistochemistry (IHC) in routinely processed celloidin- embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.",

keywords = "Antigen retrieval, Celloidin-embedding tissue, Human temporal bone, Immunohistochemistry, Microwave, Otopathology",

author = "Shi, {Shan Rong} and Cote, {Richard J.} and Taylor, {Clive R.}",

note = "Funding Information: Supported by the University Pathology Associates of the University of Southern California, the NIH Grant of CA70903-03, and the American Cancer Society (ACS IN-21-31).",

year = "1998",

month = dec,

doi = "10.1016/S0385-8146(98)00042-X",

language = "English",

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journal = "Auris Nasus Larynx",

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T2 - Standardization and development

AU - Shi, Shan Rong

AU - Cote, Richard J.

AU - Taylor, Clive R.

N1 - Funding Information: Supported by the University Pathology Associates of the University of Southern California, the NIH Grant of CA70903-03, and the American Cancer Society (ACS IN-21-31).

PY - 1998/12

Y1 - 1998/12

N2 - The use of immunohistochemistry (IHC) in routinely processed celloidin- embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.

AB - The use of immunohistochemistry (IHC) in routinely processed celloidin- embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.

KW - Antigen retrieval

KW - Celloidin-embedding tissue

KW - Human temporal bone

KW - Immunohistochemistry

KW - Microwave

KW - Otopathology

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U2 - 10.1016/S0385-8146(98)00042-X

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JO - Auris Nasus Larynx

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ER -

Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: Standardization and development

Abstract

Keywords

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