Abstract
The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a 'test battery' and the concept of 'maximal retrieval' applied to the selection of optimal test protocols for the standardization of AR.
Original language | English |
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Pages (from-to) | 327-343 |
Number of pages | 17 |
Journal | Journal of Histochemistry and Cytochemistry |
Volume | 45 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1997 |
Keywords
- antigen retrieval
- antigen-antibody recognition
- formaldehyde
- immunohistochemistry
- microwave
- protein conformation
- tissue fixation