Antigen presentation: Lysoyme, autoimmune diabetes, and listeria - What do they have in common?

Emil Unanue, Craig Byersdorfer, Javier Carrero, Matteo Levisetti, Scott Lovitch, Zheng Pu, Anish Suri

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations

Abstract

We discuss three areas of antigen presentation and macrophage biology being investigated in the laboratory. Using hen egg-white lysozyme as a protein antigen, all the segments of the molecules selected by the class II histocompatibility molecule I-Ak were identified and characterized. The display of each family of peptides was explained biochemically and quantitated. Conformational isomers of a peptide-major histocompatibility complex (MHC) complex were identified. The relationship between the amounts of peptide-MHC displayed by the antigen-presenting cells and two biologic responses, central thymic selection and T-cell responses after immunization in adjuvant, were examined. The class II MHC molecule of the nonobese diabetic I-Ag7 is being examined for its properties of peptide selection. The objective is to identify the diabetogenic peptides, as well as the repertoire of protein antigens from β-cells that trigger autoantibodies. The I-Ag7 molecule selects peptides that show very distinctive sequence motifs: one or more acidic residues at the carboxy terminus that interact at the P9 pocket of the binding groove. Finally, the investigations in listeriosis examined the early events in immune induction. More important, we found that Listeria causes marked apoptosis of lymphocytes around infective foci resulting from the apoptogenic properties of the pore-forming molecule Listeriolysin O.

Original languageEnglish
Pages (from-to)267-292
Number of pages26
JournalImmunologic Research
Volume32
Issue number1-3
DOIs
StatePublished - 2005

Keywords

  • Antigen presentation
  • Antigen processing
  • Diabetes mellitus
  • Histocompatibility molecules
  • Listeria monocytogenes
  • Lymphocyte apoptosis
  • Nonobese diabetic mouse

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