Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells

Takaharu Okada, Mark J. Miller, Ian Parker, Matthew F. Krummel, Margaret Neighbors, Suzanne B. Hartley, Anne O'Garra, Michael D. Cahalan, Jason G. Cyster

Research output: Contribution to journalArticle

388 Scopus citations

Abstract

Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 μm/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell-T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell-dependent antibody responses.

Original languageEnglish
Pages (from-to)1047-1061
Number of pages15
JournalPLoS biology
Volume3
Issue number6
DOIs
StatePublished - Jun 2005

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    Okada, T., Miller, M. J., Parker, I., Krummel, M. F., Neighbors, M., Hartley, S. B., O'Garra, A., Cahalan, M. D., & Cyster, J. G. (2005). Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. PLoS biology, 3(6), 1047-1061. https://doi.org/10.1371/journal.pbio.0030150