TY - JOUR
T1 - Antibody responses to vaccinia membrane proteins after smallpox vaccination
AU - Lawrence, Steven J.
AU - Lottenbach, Kathleen R.
AU - Newman, Frances K.
AU - Buller, R. Mark L.
AU - Bellone, Clifford J.
AU - Chen, John J.
AU - Cohen, Gary H.
AU - Eisenberg, Roselyn J.
AU - Belshe, Robert B.
AU - Stanley, Samuel L.
AU - Frey, Sharon E.
N1 - Funding Information:
Financial support: National Institutes of Health (grant 1 U54 AI057160-01 [Midwest Regional Center of Excellence {MRCE} for Biodefense and Emerging Infectious Diseases Research] to S.L.S., contract N01-AI-25464 to R.B.B. and S.E.F., and grants U54 AI57168 and 1 UC1 AI062486 to G.H.C. and R.J.E). S.J.L. was a MRCE Biodefense Clinical/Translational Fellow.
PY - 2007/7/15
Y1 - 2007/7/15
N2 - Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26-30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P < .001), whereas anti-L1 antibody responses were less robust (detection rate, 31%; GMT, 75) in nonnaive subjects. Diluted vaccine elicited antibody responses that were similar to those elicited by undiluted vaccine. Conclusions. Vaccination with VV elicits long-lived specific antibody responses directed against VV membrane proteins that vary by previous vaccination status but not with respect to 10-fold dilution of vaccine. B5, A33, and A27 should be considered for inclusion in future human orthopoxvirus subunit vaccines.
AB - Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26-30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P < .001), whereas anti-L1 antibody responses were less robust (detection rate, 31%; GMT, 75) in nonnaive subjects. Diluted vaccine elicited antibody responses that were similar to those elicited by undiluted vaccine. Conclusions. Vaccination with VV elicits long-lived specific antibody responses directed against VV membrane proteins that vary by previous vaccination status but not with respect to 10-fold dilution of vaccine. B5, A33, and A27 should be considered for inclusion in future human orthopoxvirus subunit vaccines.
UR - http://www.scopus.com/inward/record.url?scp=34447279632&partnerID=8YFLogxK
U2 - 10.1086/518793
DO - 10.1086/518793
M3 - Article
C2 - 17570109
AN - SCOPUS:34447279632
SN - 0022-1899
VL - 196
SP - 220
EP - 229
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -