Antibody Nucleic Acid Complexes. Immunospecific Retention of N6-Methyladenosine-Containing Transfer Ribonucleic Acid†

Theodore W. Munns, M. Kathryn Liszewski, Robert J. Oberst, Harold F. Sims

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11 Scopus citations

Abstract

Antibodies specific for N6-methyladenosine (m6A) were immobilized on Sepharose and the resulting immunoadsorbent was tested for its ability to retain those Escherichia coli tRNAs containing the antigenic hapten, i.e., m6A. Results obtained with [32P]PO4- and [methyl-3H]-methionine-labeled tRNAs indicated that approximately 3 to 5% of the radioactive RNA was retained by the immunoadsorbent. Under identical conditions, but in the presence of m6A (1 mg/mL), less than 0.2% of the radioactivity was retained. Subsequent characterization of the retained tRNA via (a) analysis of wethyl-3H-labeled, methylated nucleosides, (b) two-dimensional gel electrophoresis, and (c) analysis of the retention of [3H]aminoacyl-tRNA species led to the conclusion that the anti-m6A/Sepharose adsorbent quantitatively and exclusively retained a single tRNA species containing m6A, namely, tRNAVal.

Original languageEnglish
Pages (from-to)2573-2578
Number of pages6
JournalBiochemistry
Volume17
Issue number13
DOIs
StatePublished - 1978

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