TY - JOUR
T1 - Angiotensin (A I, A II, A III) receptor characterization. Correlation of prostaglandin release with peptide degradation
AU - Blumberg, A. L.
AU - Nishikawa, K.
AU - Denny, S. E.
AU - Marshall, G. R.
AU - Needleman, P.
PY - 1977
Y1 - 1977
N2 - The authors examined the ability of the angiotensins (A I, A II, A III) to release a prostagldnin E (PGE)-like substance in the isolated Krebs' perfused kidney and mesenteric vasculature of the rabbit by parallel bioassay. In the kidney, the order of potency for PGE release was A II> A III>A I with ED50's of 36, 100, and 500 pmol, respectively. In the mesenteric preparation, on the other hand, the order of potency was A III>A II>A I with ED50's of 75, 125, and 500 pmol, respectively. During one transit through the kidney 72-76% of bioassayable A I and A II was degraded. A III was 89% metabolized. In contrast, the mesenteric vasculature inactivated only 27% of A II and 23% of A III. This data suggests an inverse relationship between renal peptide degradation and PGE release. For characterization of the renal angiotensin receptor-mediating PGE release, dissociation constants (K(B)) of the competitive angiotensin antagonists [Ile7]-A III and [Sar1, Ile8]-A II were determined with each angiotensin. K(B) values of the individual antagonists were not significantly different with A I, A II, or A III; this finding suggests that one renal angiotensin receptor is involved with PGE release.
AB - The authors examined the ability of the angiotensins (A I, A II, A III) to release a prostagldnin E (PGE)-like substance in the isolated Krebs' perfused kidney and mesenteric vasculature of the rabbit by parallel bioassay. In the kidney, the order of potency for PGE release was A II> A III>A I with ED50's of 36, 100, and 500 pmol, respectively. In the mesenteric preparation, on the other hand, the order of potency was A III>A II>A I with ED50's of 75, 125, and 500 pmol, respectively. During one transit through the kidney 72-76% of bioassayable A I and A II was degraded. A III was 89% metabolized. In contrast, the mesenteric vasculature inactivated only 27% of A II and 23% of A III. This data suggests an inverse relationship between renal peptide degradation and PGE release. For characterization of the renal angiotensin receptor-mediating PGE release, dissociation constants (K(B)) of the competitive angiotensin antagonists [Ile7]-A III and [Sar1, Ile8]-A II were determined with each angiotensin. K(B) values of the individual antagonists were not significantly different with A I, A II, or A III; this finding suggests that one renal angiotensin receptor is involved with PGE release.
UR - http://www.scopus.com/inward/record.url?scp=0017645934&partnerID=8YFLogxK
U2 - 10.1161/01.RES.41.2.154
DO - 10.1161/01.RES.41.2.154
M3 - Article
C2 - 194728
AN - SCOPUS:0017645934
VL - 41
SP - 154
EP - 158
JO - Unknown Journal
JF - Unknown Journal
IS - 2
ER -