Angiotensin (A I, A II, A III) receptor characterization. Correlation of prostaglandin release with peptide degradation

A. L. Blumberg, K. Nishikawa, S. E. Denny, G. R. Marshall, P. Needleman

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The authors examined the ability of the angiotensins (A I, A II, A III) to release a prostagldnin E (PGE)-like substance in the isolated Krebs' perfused kidney and mesenteric vasculature of the rabbit by parallel bioassay. In the kidney, the order of potency for PGE release was A II> A III>A I with ED50's of 36, 100, and 500 pmol, respectively. In the mesenteric preparation, on the other hand, the order of potency was A III>A II>A I with ED50's of 75, 125, and 500 pmol, respectively. During one transit through the kidney 72-76% of bioassayable A I and A II was degraded. A III was 89% metabolized. In contrast, the mesenteric vasculature inactivated only 27% of A II and 23% of A III. This data suggests an inverse relationship between renal peptide degradation and PGE release. For characterization of the renal angiotensin receptor-mediating PGE release, dissociation constants (K(B)) of the competitive angiotensin antagonists [Ile7]-A III and [Sar1, Ile8]-A II were determined with each angiotensin. K(B) values of the individual antagonists were not significantly different with A I, A II, or A III; this finding suggests that one renal angiotensin receptor is involved with PGE release.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalUnknown Journal
Volume41
Issue number2
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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