TY - CHAP
T1 - Analytical Ultracentrifugation Analysis of the Self-Association of κ-Bungarotoxin
AU - Fiordalisi, James J.
AU - Grant, Gregory A.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - This chapter describes the analytical ultracentrifugation analysis of the self-association of κ–bungarotoxin. κ-bungarotoxin is physiologically active in the 200 to 400 nM range, expressed as monomer concentration. The chapter presents the sedimentation equilibrium profile obtained for both α- and κ-bungarotoxin. The sedimentation data for α-bungarotoxin is exactly superimposable on a theoretical curve for a single noninteracting species, thus, indicating that α-bungarotoxin exists strictly in the monomeric state at these concentrations. When the molecular weight parameter is unconstrained in the data analysis, an experimentally determined molecular weight of 7562 is determined. This agrees favorably with an actual molecular weight of 7984. The data for κ-bungarotoxin falls part way between two theoretical curves, one for a single noninteracting species and another for the completely associated dimer.
AB - This chapter describes the analytical ultracentrifugation analysis of the self-association of κ–bungarotoxin. κ-bungarotoxin is physiologically active in the 200 to 400 nM range, expressed as monomer concentration. The chapter presents the sedimentation equilibrium profile obtained for both α- and κ-bungarotoxin. The sedimentation data for α-bungarotoxin is exactly superimposable on a theoretical curve for a single noninteracting species, thus, indicating that α-bungarotoxin exists strictly in the monomeric state at these concentrations. When the molecular weight parameter is unconstrained in the data analysis, an experimentally determined molecular weight of 7562 is determined. This agrees favorably with an actual molecular weight of 7984. The data for κ-bungarotoxin falls part way between two theoretical curves, one for a single noninteracting species and another for the completely associated dimer.
UR - http://www.scopus.com/inward/record.url?scp=85013522166&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-194710-1.50035-7
DO - 10.1016/B978-0-12-194710-1.50035-7
M3 - Chapter
AN - SCOPUS:85013522166
T3 - Techniques in Protein Chemistry
SP - 269
EP - 274
BT - Techniques in Protein Chemistry
ER -