Analytical Ultracentrifugation Analysis of the Self-Association of κ-Bungarotoxin

James J. Fiordalisi, Gregory A. Grant

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations


This chapter describes the analytical ultracentrifugation analysis of the self-association of κ–bungarotoxin. κ-bungarotoxin is physiologically active in the 200 to 400 nM range, expressed as monomer concentration. The chapter presents the sedimentation equilibrium profile obtained for both α- and κ-bungarotoxin. The sedimentation data for α-bungarotoxin is exactly superimposable on a theoretical curve for a single noninteracting species, thus, indicating that α-bungarotoxin exists strictly in the monomeric state at these concentrations. When the molecular weight parameter is unconstrained in the data analysis, an experimentally determined molecular weight of 7562 is determined. This agrees favorably with an actual molecular weight of 7984. The data for κ-bungarotoxin falls part way between two theoretical curves, one for a single noninteracting species and another for the completely associated dimer.

Original languageEnglish
Title of host publicationTechniques in Protein Chemistry
Number of pages6
StatePublished - Jan 1 1994

Publication series

NameTechniques in Protein Chemistry
ISSN (Print)1080-8914


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