TY - JOUR
T1 - Analytical titration curves of glycosyl hydrolase Cel45 by combined isoelectric focusing - Electrophoresis
AU - Attanasio, Francesco
AU - Bruschi, Maurizio
AU - Candiano, Giovanni
AU - Galletto, Roberto
AU - Musante, Luca
AU - Schülein, Martin
AU - Rialdi, Giovanni
PY - 1999
Y1 - 1999
N2 - The electrical charge of endocellulase Cel45-core has been determined by combined isoelectric focusing-electrophoresis in the range of pH 3-9. In order to transform electrophoretic mobility to absolute electrical charge value, several corrections were applied: the frictional coefficient theoretically calculated from the molecular dimensions depends on porous gel structure and on the ionic strength of the solution. By comparing the curve calculated according to the Linderstrom-Lang equation, the number of charged electrical groups exposed to the solvent and their apparent ionization constants, pK(o)(i), can be determined. Furthermore, the macromolecule structure can be assumed not to change in this pH range. This finding is necessary to understand the structure and the electrical properties of the entire Cel45 molecule.
AB - The electrical charge of endocellulase Cel45-core has been determined by combined isoelectric focusing-electrophoresis in the range of pH 3-9. In order to transform electrophoretic mobility to absolute electrical charge value, several corrections were applied: the frictional coefficient theoretically calculated from the molecular dimensions depends on porous gel structure and on the ionic strength of the solution. By comparing the curve calculated according to the Linderstrom-Lang equation, the number of charged electrical groups exposed to the solvent and their apparent ionization constants, pK(o)(i), can be determined. Furthermore, the macromolecule structure can be assumed not to change in this pH range. This finding is necessary to understand the structure and the electrical properties of the entire Cel45 molecule.
KW - Electrophoretic titraton curves
KW - Endoglucanase
UR - http://www.scopus.com/inward/record.url?scp=0033039582&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1522-2683(19990601)20:7<1403::AID-ELPS1403>3.0.CO;2-6
DO - 10.1002/(SICI)1522-2683(19990601)20:7<1403::AID-ELPS1403>3.0.CO;2-6
M3 - Article
C2 - 10424462
AN - SCOPUS:0033039582
SN - 0173-0835
VL - 20
SP - 1403
EP - 1411
JO - Electrophoresis
JF - Electrophoresis
IS - 7
ER -