@article{2464c67633374b48b052c38c41418f4a,
title = "Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock",
abstract = "This study employs biomass growth analyses and 13C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock.",
keywords = "C-isotope tracing, Catabolite repression, Dimorphism, Metabolic network, β-carotene",
author = "Worland, {Alyssa M.} and Czajka, {Jeffrey J.} and Yun Xing and Harper, {Willie F.} and Aryiana Moore and Zhengyang Xiao and Zhenlin Han and Yechun Wang and Su, {Wei Wen} and Tang, {Yinjie J.}",
note = "Funding Information: AMW and JJC were supported by Arch Innotek in St. Louis via an NIH small business grant (1R41GM13027701). LC-MS data acquisition was performed at the Proteomics and Mass Spectrometry Core Facility at the Donald Danforth Plant Science Center in St. Louis. This facility is funded by the NSF (DBI1427621). WWS and ZLH acknowledge support from the National Institute of Food and Agriculture (Hatch project HAW05040-H, Multistate project HAW05041-R) and NIFA grant (2020-67022-31146). Funding Information: AMW and JJC were supported by Arch Innotek in St. Louis via an NIH small business grant ( 1R41GM13027701 ). LC-MS data acquisition was performed at the Proteomics and Mass Spectrometry Core Facility at the Donald Danforth Plant Science Center in St. Louis. This facility is funded by the NSF ( DBI1427621 ). WWS and ZLH acknowledge support from the National Institute of Food and Agriculture (Hatch project HAW05040-H, Multistate project HAW05041-R ) and NIFA grant ( 2020-67022-31146 ). Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = dec,
doi = "10.1016/j.mec.2020.e00130",
language = "English",
volume = "11",
journal = "Metabolic Engineering Communications",
issn = "2214-0301",
}