TY - JOUR
T1 - Analysis of the herpes simplex virus type 1 UL6 gene in patients with stromal keratitis
AU - Ellison, Aaron R.
AU - Yang, Li
AU - Cevallos, A. Vicky
AU - Margolis, Todd P.
N1 - Funding Information:
We thank Y. Jerold Gordon and Regis Kowalski for clinical isolates of HSV-1 from the Campbell Microbiology lab, David Bloom for HSV-1 strains ANG and McKrae, and Brad Mitchell for HSV-1 strain HFEM. This work was supported by National Institutes of Health Grants EY10008 and EY02162, a Research to Prevent Blindness Lew Wasserman Merit Award (New York, NY), and the Ralph and Sophie Heintz Laboratory Fund (San Francisco, CA).
PY - 2003/5/25
Y1 - 2003/5/25
N2 - Recent work suggests that herpes simplex virus (HSV) stromal keratitis in the mouse is caused by autoreactive T lymphocytes triggered by a 16 amino acid region of the HSV UL6 protein (aa299-314) (Z.-S. Zhao, F. Granucci, L. Yeh, P. Schaffer, H. Cantor, 1998, Science 279, 1344-1347). In the present study we sought to determine whether genetic variation of this presumed autoreactive UL6 epitope is responsible for different pathogenic patterns of human HSV keratitis. To accomplish this, we sequenced the HSV UL6 gene from ocular isolates of 10 patients with necrotizing stromal keratitis, 7 patients with recurrent epithelial keratitis, and 8 patients with other forms of HSV keratitis. The sequences obtained predicted identical UL6(299-314) epitopes for all 25 viral isolates. Furthermore, the upstream sequence of all isolates was free of insertions, deletions, and stop codons. We conclude that different pathogenic patterns of human HSV keratitis occur independent of genetic variation of the HSV UL6 (299-314) epitope.
AB - Recent work suggests that herpes simplex virus (HSV) stromal keratitis in the mouse is caused by autoreactive T lymphocytes triggered by a 16 amino acid region of the HSV UL6 protein (aa299-314) (Z.-S. Zhao, F. Granucci, L. Yeh, P. Schaffer, H. Cantor, 1998, Science 279, 1344-1347). In the present study we sought to determine whether genetic variation of this presumed autoreactive UL6 epitope is responsible for different pathogenic patterns of human HSV keratitis. To accomplish this, we sequenced the HSV UL6 gene from ocular isolates of 10 patients with necrotizing stromal keratitis, 7 patients with recurrent epithelial keratitis, and 8 patients with other forms of HSV keratitis. The sequences obtained predicted identical UL6(299-314) epitopes for all 25 viral isolates. Furthermore, the upstream sequence of all isolates was free of insertions, deletions, and stop codons. We conclude that different pathogenic patterns of human HSV keratitis occur independent of genetic variation of the HSV UL6 (299-314) epitope.
KW - Autoimmune response/disease
KW - Herpes simplex virus
KW - Immunopathology
KW - Keratitis
KW - Molecular mimicry
KW - UL6
KW - Virus infection
UR - http://www.scopus.com/inward/record.url?scp=0037867922&partnerID=8YFLogxK
U2 - 10.1016/S0042-6822(03)00075-8
DO - 10.1016/S0042-6822(03)00075-8
M3 - Article
C2 - 12788627
AN - SCOPUS:0037867922
SN - 0042-6822
VL - 310
SP - 24
EP - 28
JO - Virology
JF - Virology
IS - 1
ER -