Analysis of libraries encoded with GC tags: Compound elution, tag decode analysis, and statistical sampling analysis

Roland E. Dolle; Joan Guo; Wenni Li; Ning Zhao; James A. Connelly

doi:10.1023/A:1011393414573

Analysis of libraries encoded with GC tags: Compound elution, tag decode analysis, and statistical sampling analysis

Roland E. Dolle, Joan Guo, Wenni Li, Ning Zhao, James A. Connelly

Center for Drug Discovery

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Libraries encoded with electrophoric tags present a unique challenge with respect to library quality control and characterization. Libraries are prepared on Tentagel resin in 200-fold redundancy wherein each resin particle contains one compound per one tag set. The amount of compound present on the bead is ca. 200-500 pmole while tag levels are estimated at 0.5-1 pmol/bead. Several quality control protocols have been developed in order to accurately estimate bead yield and purity for the entire library, ensure high tag fidelity, and to determine the overall performance of individual synthons. This review provides a unique, collective portrait of Pharmacopeia's approach in assessing the quality of libraries prepared using its molecular encoding technology.

Original language	English
Pages (from-to)	35-49
Number of pages	15
Journal	Molecular Diversity
Volume	5
Issue number	1
DOIs	https://doi.org/10.1023/A:1011393414573
State	Published - 2000

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Analysis of libraries encoded with GC tags: Compound elution, tag decode analysis, and statistical sampling analysis

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