TY - JOUR
T1 - Analysis of kinetic data
T2 - Practical applications of computer simulation and fitting programs
AU - Frieden, Carl
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The correct analysis of experimental data is central to understanding the underlying mechanism of any kinetic process. The advent of computer programs that can numerically solve differential equations easily and the development of computers that can run those programs rapidly have changed the perspective regarding the analysis of kinetic data. It is now possible to simulate rapidly the time course of kinetically complex systems and to obtain not only the time dependence of reactant disappearance or product appearance but also the concentration of any or all intermediates in the pathway. It is possible to optimize rate constants for steps in the process using best fit procedures. This chapter reviews various applications of one of the programs used in data analysis, the way in which the programs can be used, and the type of information that can be obtained. The chapter discusses the programs for a virtual address extension (VAX) system, in either its original (virtual memory system) or modified form using graphical Kernal system (GKS) graphics, or that have been adapted to run on a personal computer (PC), on a Macintosh, or in a Silicon Graphics environment.
AB - The correct analysis of experimental data is central to understanding the underlying mechanism of any kinetic process. The advent of computer programs that can numerically solve differential equations easily and the development of computers that can run those programs rapidly have changed the perspective regarding the analysis of kinetic data. It is now possible to simulate rapidly the time course of kinetically complex systems and to obtain not only the time dependence of reactant disappearance or product appearance but also the concentration of any or all intermediates in the pathway. It is possible to optimize rate constants for steps in the process using best fit procedures. This chapter reviews various applications of one of the programs used in data analysis, the way in which the programs can be used, and the type of information that can be obtained. The chapter discusses the programs for a virtual address extension (VAX) system, in either its original (virtual memory system) or modified form using graphical Kernal system (GKS) graphics, or that have been adapted to run on a personal computer (PC), on a Macintosh, or in a Silicon Graphics environment.
UR - http://www.scopus.com/inward/record.url?scp=0028673742&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(94)40053-9
DO - 10.1016/S0076-6879(94)40053-9
M3 - Article
C2 - 7823836
AN - SCOPUS:0028673742
VL - 240
SP - 311
EP - 322
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -