Analysis of kinetic data: Practical applications of computer simulation and fitting programs

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Abstract

The correct analysis of experimental data is central to understanding the underlying mechanism of any kinetic process. The advent of computer programs that can numerically solve differential equations easily and the development of computers that can run those programs rapidly have changed the perspective regarding the analysis of kinetic data. It is now possible to simulate rapidly the time course of kinetically complex systems and to obtain not only the time dependence of reactant disappearance or product appearance but also the concentration of any or all intermediates in the pathway. It is possible to optimize rate constants for steps in the process using best fit procedures. This chapter reviews various applications of one of the programs used in data analysis, the way in which the programs can be used, and the type of information that can be obtained. The chapter discusses the programs for a virtual address extension (VAX) system, in either its original (virtual memory system) or modified form using graphical Kernal system (GKS) graphics, or that have been adapted to run on a personal computer (PC), on a Macintosh, or in a Silicon Graphics environment.

Original languageEnglish
Pages (from-to)311-322
Number of pages12
JournalMethods in enzymology
Volume240
Issue numberC
DOIs
StatePublished - Jan 1 1994

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