Analysis of human cellular retinol-binding protein II promoter during enterocyte differentiation

Liang Zhang, E. Xueping, Kathryn E. Luker, Jian Su Shao, Marc S. Levin, Eunran Suh, Ellen Li

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9 Scopus citations

Abstract

Cellular retinol binding protein II (CRBP II) is a vitamin A-binding protein that is expressed specifically in small intestinal villus absorptive cells. Previous studies have shown that retinoic acid upregulates endogenous human CRBP II gene expression in differentiated Caco-2 cells. To better characterize the regulation of human CRBP II expression, we analyzed the ability of receptor-selective agonists to enhance transcription from the 5′-upstream flanking region of the human CRBP II gene. Stable transfection experiments showed that the proximal 2.8-kb region of the human CRBP II gene is sufficient for retinoic acid inducibility in differentiated Caco-2 cells. However, direct sequence analysis and transient transfection experiments indicate that, unlike the rat CRBP II promoter, the human CRBP II promoter is not a direct retinoid X receptor target. The results indicate that the retinoic acid responsiveness of the human CRBP II promoter is mediated by an indirect mechanism and that this mechanism is associated with enterocyte differentiation.

Original languageEnglish
Pages (from-to)G1079-G1087
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume282
Issue number6 45-6
DOIs
StatePublished - 2002

Keywords

  • Caco-2 cells
  • Gene regulation
  • Retinoid X receptor
  • Vitamin A

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