Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Chloe Potter, Didier Hodzic

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The biochemical characterization of proteins most often require their identification by immunoblotting. Whereas SDS-PAGE provides satisfactory results for most proteins, the identification of larger proteins requires alternative methods to ensure their separation and complete transfer onto nitrocellulose membranes. Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-33
Number of pages9
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1840
ISSN (Print)1064-3745

Keywords

  • LINC complexes
  • Nesprin-1 giant
  • Nesprin-2 giant
  • Nesprins
  • SUN proteins
  • VAGE
  • Vertical agarose gel electrophoresis

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  • Cite this

    Potter, C., & Hodzic, D. (2018). Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis. In Methods in Molecular Biology (pp. 25-33). (Methods in Molecular Biology; Vol. 1840). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8691-0_3