@inbook{40b36bd80ae746a8bbcd98dc13bfee42,
title = "Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis",
abstract = "The biochemical characterization of proteins most often require their identification by immunoblotting. Whereas SDS-PAGE provides satisfactory results for most proteins, the identification of larger proteins requires alternative methods to ensure their separation and complete transfer onto nitrocellulose membranes. Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2.",
keywords = "LINC complexes, Nesprin-1 giant, Nesprin-2 giant, Nesprins, SUN proteins, VAGE, Vertical agarose gel electrophoresis",
author = "Chloe Potter and Didier Hodzic",
note = "Funding Information: This work was funded by an NIH grant from the National Eye Institute (#EY022632 to D.H.) and an unrestricted grant to the Department of Ophthalmology and Visual Sciences at Washington University from Research to Prevent Blindness. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8691-0_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--33",
booktitle = "Methods in Molecular Biology",
}