TY - JOUR
T1 - Analysis of genetic variability in Aristaeomorpha foliacea (Crustacea, Aristeidae) using DNA-ISSR (Inter Simple Sequence Repeats) markers
AU - Fernández, Maria Victoria
AU - Maltagliati, Ferruccio
AU - Pannacciulli, Federica G.
AU - Roldán, Maria Inés
N1 - Funding Information:
Authors wish to thank A.M. Carbonell, K. Kapiris, C.Y. Politou, I. Sobrino, A. Souplet and A. Turco for their help in sample collection and P. Cossu for his technical assistance. Two reviewers provided constructive comments on the manuscript. This work was supported by a grant to MIR by Ministerio de Ciencia e Innovación, Spain (AGL2009-09228) and by a predoctoral fellowship to MVF by Ministerio de Educación y Ciencia, Spain (BES-2007-15865).
PY - 2011/10
Y1 - 2011/10
N2 - This work reports the first genetic data of Aristaeomorpha foliacea, a marine decapod of high commercial value, from six Mediterranean localities and one new fishing ground in the Mozambique Channel. The use of five Inter Simple Sequence Repeat (ISSR) primers provided 150 polymorphic loci. Average estimates of genetic diversity did not significantly differ among sampled localities, with a mean value of heterozygosity H = 0.105 ± 0.015. Analysis of molecular variance (AMOVA) allocated > 98% of genetic variability to the within-sample component, displaying values higher than those previously reported in ISSR studies on marine invertebrates. Cluster analyses did not detect geographically or genetically distinct groups. The observed lack of large-scale genetic differentiation is discussed in relation to the high potential for larval dispersal of the species and to features of the marker employed.
AB - This work reports the first genetic data of Aristaeomorpha foliacea, a marine decapod of high commercial value, from six Mediterranean localities and one new fishing ground in the Mozambique Channel. The use of five Inter Simple Sequence Repeat (ISSR) primers provided 150 polymorphic loci. Average estimates of genetic diversity did not significantly differ among sampled localities, with a mean value of heterozygosity H = 0.105 ± 0.015. Analysis of molecular variance (AMOVA) allocated > 98% of genetic variability to the within-sample component, displaying values higher than those previously reported in ISSR studies on marine invertebrates. Cluster analyses did not detect geographically or genetically distinct groups. The observed lack of large-scale genetic differentiation is discussed in relation to the high potential for larval dispersal of the species and to features of the marker employed.
KW - Aristaeomorpha foliacea
KW - DNA-ISSRs
KW - Gene flow
KW - Genetic variability
KW - Marine decapod
UR - http://www.scopus.com/inward/record.url?scp=80053339663&partnerID=8YFLogxK
U2 - 10.1016/j.crvi.2011.07.005
DO - 10.1016/j.crvi.2011.07.005
M3 - Article
C2 - 21943519
AN - SCOPUS:80053339663
SN - 1631-0691
VL - 334
SP - 705
EP - 712
JO - Comptes Rendus - Biologies
JF - Comptes Rendus - Biologies
IS - 10
ER -