38 Scopus citations


After partial resection, the remnant small intestine undergoes an adaptive response. Little is known about the molecular and cellular basis of intestinal adaptation. To identify genes transcriptionally regulated in response to loss of functional bowel surface area, we have isolated cDNAs differentially expressed in the adaptive ileum 48 h after 70% proximal small intestinal resection. A cDNA library constructed from the remnant ileum of rats subjected to resection was screened using subtractive hybridization techniques. Several groups of cDNA clones that were induced during intestinal adaptation were isolated. The first included liver fatty acid binding protein, apolipoprotein A-IV, cellular retinol binding protein I1, and ileal lipid binding protein. These all encode proteins involved in the absorption, metabolism, and trafficking of nutrients. A second group included the catalytic subunit of protein phosphatase 1δ, a 78-kDa glucose-regulated protein (grp78; a glucose-regulated member of the 70-kDa heat-shock protein family), and several pancreatitis-associated proteins. A third group of induced genes contained novel cDNAs. To better characterize the adaptive response, the temporal, spatial, and cellular patterns of expression of several of these genes were analyzed with the use of immunohistochemical and in situ hybridization techniques. These studies indicate that during early adaptation, genes involved in nutrient trafficking, protein processing, and cell cycle regulation are transcriptionally regulated in the residual small intestine in distinct temporal and regional patterns consistent with a complex multifaceted response to intestinal resection.

Original languageEnglish
Pages (from-to)G347-G356
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number2 34-2
StatePublished - Aug 1996


  • 78-kilodalton glucose-regulated protein
  • apolipoprotein A-IV
  • cellular retinol binding protein
  • fatty acid binding protein
  • ileal lipid binding protein
  • intestinal adaptation
  • pancreatitis-associated protein
  • protein phosphatase 1δ
  • protein phosphatase I


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