TY - JOUR
T1 - Analysis of carotenoid isomerase activity in a prototypical carotenoid cleavage enzyme, apocarotenoid oxygenase (ACO)
AU - Sui, Xuewu
AU - Kiser, Philip D.
AU - Che, Tao
AU - Carey, Paul R.
AU - Golczak, Marcin
AU - Shi, Wuxian
AU - Von Lintig, Johannes
AU - Palczewski, Krzysztof
PY - 2014
Y1 - 2014
N2 - Background: Isomerase activity for Synechocystis apocarotenoid oxygenase (ACO) has not been verified biochemically. Results: ACO exclusively produces all-trans-retinal from all-trans-apo-8β-cells-carotenol and is inhibited by linear polyoxyethylene detergents. Conclusion: ACO cleaves but does not isomerize all-trans-8β-cells-apocarotenol. Significance: This study clarifies the activity of and influence of detergent on ACO, a classical non-isomerizing member of the carotenoid cleavage enzyme family.
AB - Background: Isomerase activity for Synechocystis apocarotenoid oxygenase (ACO) has not been verified biochemically. Results: ACO exclusively produces all-trans-retinal from all-trans-apo-8β-cells-carotenol and is inhibited by linear polyoxyethylene detergents. Conclusion: ACO cleaves but does not isomerize all-trans-8β-cells-apocarotenol. Significance: This study clarifies the activity of and influence of detergent on ACO, a classical non-isomerizing member of the carotenoid cleavage enzyme family.
UR - https://www.scopus.com/pages/publications/84899751897
U2 - 10.1074/jbc.M114.552836
DO - 10.1074/jbc.M114.552836
M3 - Article
C2 - 24648526
AN - SCOPUS:84899751897
SN - 0021-9258
VL - 289
SP - 12286
EP - 12299
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 18
ER -