Analysis of adult otitis media: Polymerase chain reaction versus culture for bacteria and viruses

J. Christopher Post, Gregory J. White, Eric M. Llederman, Jerome J. Aul, Craig A. Buchman, D. Alexa Sirko, Garth D. Ehrlich

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Recent studies using the polymerase chain reaction (PCR) have identified bacterial and viral genomic sequences in culture-negative pediatric middle ear effusions. To evaluate this technique in adults, 19 effusions were analyzed to compare bacterial and viral culture and PCR detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and adenovirus. Effusions from 4 subjects positive for human immunodeficiency virus (HIV) were analyzed by PCR for HIV virus. Three of 19 effusions were culture-positive for bacteria, and 0 of 19 for viruses. Fifteen of 19 effusions were PCR-positive for bacterial genomic sequences, and 0 of 19 for adenovirus. Thirteen of 15 PCR-positive specimens demonstrated S pneumoniae, 5 of 15 H influenzae, and 0 of 13 M catarrhalis. All 4 effusions from HIV- positive subjects were PCR-positive for HIV. No effusion was culture-positive arid PCR-negative. These results confirm that culture-negative middle ear effusions contain genomic sequences from bacterial pathogens. Finding of HIV RNA and DNA in effusion from HIV-positives suggests replicating virus in this fluid.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalAnnals of Otology, Rhinology and Laryngology
Volume107
Issue number1
StatePublished - Jan 29 1998
Externally publishedYes

Keywords

  • Bacteria
  • Culture
  • Otitis media
  • Polymerase chain reaction
  • Virus

Fingerprint Dive into the research topics of 'Analysis of adult otitis media: Polymerase chain reaction versus culture for bacteria and viruses'. Together they form a unique fingerprint.

  • Cite this

    Christopher Post, J., White, G. J., Llederman, E. M., Aul, J. J., Buchman, C. A., Alexa Sirko, D., & Ehrlich, G. D. (1998). Analysis of adult otitis media: Polymerase chain reaction versus culture for bacteria and viruses. Annals of Otology, Rhinology and Laryngology, 107(1), 10-16.