@article{75b666f588ad40788b5889e35f24ba63,
title = "An optimized two-finger archive for ZFN-mediated gene targeting",
abstract = "The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.",
author = "Ankit Gupta and Christensen, {Ryan G.} and Rayla, {Amy L.} and Abirami Lakshmanan and Stormo, {Gary D.} and Wolfe, {Scot A.}",
note = "Funding Information: This research was supported by the US National Institutes of Health R01GM068110 (S.A.W.), R24GM078369, R01HL093766 (N. Lawson & S.A.W.) and R01HG00249 (G.D.S.). We thank N. Lawson and members of his laboratory for their insightful advice and zebrafish husbandry training, and J. Zhu for assistance with website construction.",
year = "2012",
month = jun,
doi = "10.1038/nmeth.1994",
language = "English",
volume = "9",
pages = "588--590",
journal = "Nature Methods",
issn = "1548-7091",
number = "6",
}