An optimized two-finger archive for ZFN-mediated gene targeting

Ankit Gupta, Ryan G. Christensen, Amy L. Rayla, Abirami Lakshmanan, Gary D. Stormo, Scot A. Wolfe

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.

Original languageEnglish
Pages (from-to)588-590
Number of pages3
JournalNature Methods
Volume9
Issue number6
DOIs
StatePublished - Jun 2012

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