TY - JOUR
T1 - An intermediate in transposition of the conjugative transposon Tn916.
AU - Scott, J. R.
AU - Kirchman, P. A.
AU - Caparon, M. G.
PY - 1988/7
Y1 - 1988/7
N2 - Using the conjugative transposon Tn916, we have identified a closed covalent circular form produced in vivo that is able to serve as an intermediate in transposition. When a region of a streptococcal chromosome containing Tn916 is cloned in an Escherichia coli plasmid, supercoiled transposon molecules are excised spontaneously. The purified supercoiled forms transform Bacillus subtilis protoplasts by inserting into the chromosome, apparently at random. In B. subtilis, Tn916 retains its ability to promote conjugative transposition, as shown by its transfer to Streptococcus pyogenes.
AB - Using the conjugative transposon Tn916, we have identified a closed covalent circular form produced in vivo that is able to serve as an intermediate in transposition. When a region of a streptococcal chromosome containing Tn916 is cloned in an Escherichia coli plasmid, supercoiled transposon molecules are excised spontaneously. The purified supercoiled forms transform Bacillus subtilis protoplasts by inserting into the chromosome, apparently at random. In B. subtilis, Tn916 retains its ability to promote conjugative transposition, as shown by its transfer to Streptococcus pyogenes.
UR - http://www.scopus.com/inward/record.url?scp=0024044902&partnerID=8YFLogxK
U2 - 10.1073/pnas.85.13.4809
DO - 10.1073/pnas.85.13.4809
M3 - Article
C2 - 2838847
AN - SCOPUS:0024044902
SN - 0027-8424
VL - 85
SP - 4809
EP - 4813
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -