An inducible cell model for studies of V(D)J recombinational control

Michael L. Sikes, Eugene M. Oltz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Antigen receptor gene assembly is controlled by enhancer-directed changes in the accessibility of chromosomal gene segments to V(D)J recombinase. To dissect mechanisms that regulate rearrangement efficiencies, we developed a cell system (TDR19) in which recombination activating gene (RAG) expression is repressed by tetracycline. Under conditions of RAG repression, recombination substrates were consistently integrated into the TDR19 genome in an unrearranged form. Subsequent rearrangement of chromosomal substrates containing a transcriptional enhancer correlated inversely with tetracycline concentrations. Together, these features underscore the utility of TDR19 as a cell model for defining the molecular determinants of V(D)J recombinational accessibility.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalJournal of Immunological Methods
Volume224
Issue number1-2
DOIs
StatePublished - Apr 22 1999

Keywords

  • Inducible promoter
  • T Cell receptor
  • Transcriptional enhancers
  • V(D)J recombination

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