Abstract
Antigen receptor gene assembly is controlled by enhancer-directed changes in the accessibility of chromosomal gene segments to V(D)J recombinase. To dissect mechanisms that regulate rearrangement efficiencies, we developed a cell system (TDR19) in which recombination activating gene (RAG) expression is repressed by tetracycline. Under conditions of RAG repression, recombination substrates were consistently integrated into the TDR19 genome in an unrearranged form. Subsequent rearrangement of chromosomal substrates containing a transcriptional enhancer correlated inversely with tetracycline concentrations. Together, these features underscore the utility of TDR19 as a cell model for defining the molecular determinants of V(D)J recombinational accessibility.
Original language | English |
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Pages (from-to) | 25-29 |
Number of pages | 5 |
Journal | Journal of Immunological Methods |
Volume | 224 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 22 1999 |
Keywords
- Inducible promoter
- T Cell receptor
- Transcriptional enhancers
- V(D)J recombination