@article{37721098c73e440292fffd98f3e13be5,
title = "An Individual Interneuron Participates in Many Kinds of Inhibition and Innervates Much of the Mouse Visual Thalamus",
abstract = "One way to assess a neuron's function is to describe all its inputs and outputs. With this goal in mind, we used serial section electron microscopy to map 899 synaptic inputs and 623 outputs in one inhibitory interneuron in a large volume of the mouse visual thalamus. This neuron innervated 256 thalamocortical cells spread across functionally distinct subregions of the visual thalamus. All but one of its neurites were bifunctional, innervating thalamocortical and local interneurons while also receiving synapses from the retina. We observed a wide variety of local synaptic motifs. While this neuron innervated many cells weakly, with single en passant synapses, it also deployed specialized branches that climbed along other dendrites to form strong multi-synaptic connections with a subset of partners. This neuron's diverse range of synaptic relationships allows it to participate in a mix of global and local processing but defies assigning it a single circuit function.",
keywords = "LGN, RGC, connectomic, dLGN, electron microscopy, interneuron, lateral geniculate nucleus, local interneuron, microcircuit, mouse",
author = "Morgan, {Josh L.} and Lichtman, {Jeff W.}",
note = "Funding Information: Image alignment was performed by Art Wetzel at Pittsburgh Supercomputing (low resolution) and Adi Peleg and Shuohong Wang (high resolution). We gratefully acknowledge support from the National Eye Institute (NEI) ( 1R21EEYE030623-01 ); National Institutes of Health (NIH)/ NINDS ( 1DP2OD006514-01 , TR01 1R01NS076467-01 , 1U01NS090449-01 , and U24NS109102 ); Conte ( 1P50MH094271-01 ); MURI Army Research Office (contracts W911NF1210594 and IIS-1447786 ); NSF ( OIA-1125087 and IIS-1110955 ); the Human Frontier Science Program ( RGP0051/2014 ); and the NIH and NIGMS via the National Center for Multiscale Modeling of Biological Systems ( P41GM10371 ). J.L.M. is a recipient of a Research to Prevent Blindness Career Development Award. This work was supported by an unrestricted grant to the Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness and by the NEI/NIH under award number P30 EY002687 . We are grateful to James Cuff and Harvard Research Computing for data management. We thank Richard Schalek for microscope hardware management, Katia Valkova for tracing help, and Jordan Matelsky, Brock Wester, William Gray-Roncal, and the team at bossDB. Editing assistance was provided by InPrint at Washington University in St. Louis. Funding Information: Image alignment was performed by Art Wetzel at Pittsburgh Supercomputing (low resolution) and Adi Peleg and Shuohong Wang (high resolution). We gratefully acknowledge support from the National Eye Institute (NEI) (1R21EEYE030623-01); National Institutes of Health (NIH)/NINDS (1DP2OD006514-01, TR01 1R01NS076467-01, 1U01NS090449-01, and U24NS109102); Conte (1P50MH094271-01); MURI Army Research Office (contracts W911NF1210594 and IIS-1447786); NSF (OIA-1125087 and IIS-1110955); the Human Frontier Science Program (RGP0051/2014); and the NIH and NIGMS via the National Center for Multiscale Modeling of Biological Systems (P41GM10371). J.L.M. is a recipient of a Research to Prevent Blindness Career Development Award. This work was supported by an unrestricted grant to the Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness and by the NEI/NIH under award number P30 EY002687. We are grateful to James Cuff and Harvard Research Computing for data management. We thank Richard Schalek for microscope hardware management, Katia Valkova for tracing help, and Jordan Matelsky, Brock Wester, William Gray-Roncal, and the team at bossDB. Editing assistance was provided by InPrint at Washington University in St. Louis. Conceptualization, J.L.M. and J.W.L.; Methodology, J.L.M.; Software, J.L.M.; Formal Analysis, J.L.M. Investigation, J.L.M.; Resources, J.L.M. and J.W.L.; Data Curation, J.L.M.; Writing, J.L.M. and J.W.L.; Visualization, J.L.M.; Funding Acquisition, J.L.M. and J.W.L. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = may,
day = "6",
doi = "10.1016/j.neuron.2020.02.001",
language = "English",
volume = "106",
pages = "468--481.e2",
journal = "Neuron",
issn = "0896-6273",
number = "3",
}