TY - JOUR
T1 - An increase in the synthesis and release of calcitonin gene-related peptide in two-kidney, one-clip hypertensive rats
AU - Deng, Pan Yue
AU - Ye, Feng
AU - Zhu, Hui Qing
AU - Cai, Wei Jun
AU - Deng, Han Wu
AU - Li, Yuan Jian
N1 - Funding Information:
This work was supported by grants (No. 30070870) from National Nature Science Foundation and the Ministry of Education, PR China.
PY - 2003/7/15
Y1 - 2003/7/15
N2 - Previous investigations have indicated that capsaicin-sensitive sensory nerves play an important role in modulation of the peripheral resistance of the circulation system. In the present study, we examined the role of capsaicin-sensitive sensory nerves in two-kidney, one-clip (2K1C) renovascular hypertension rats. Systolic blood pressure (BP) was monitored by the tail-cuff method throughout the experiment. Concentrations of calcitonin gene-related peptide (CGRP) in the plasma, the level of CGRP mRNA in dorsal root ganglia (DRG) and the density of CGRP immunoreactive (CGRP-ir) fibers in mesenteric artery were measured. Blood pressure was significantly elevated at day 10 postoperation (BP was 143±10 and 114±7 mm Hg for 2K1C and Sham groups, respectively, p<0.05). Treatment with capsaicin, which selectively depletes neurotransmitters in sensory nerves, enhanced hypertensive responses to clipping (BP was 168±7 and 143±10 mm Hg at day 10 postoperation for Cap1+2K1C and 2K1C groups, respectively, p<0.05), and BP in the rats treated with a second injection of capsaicin was greater than that in the rats treated with a single injection of capsaicin (At day 30 postoperation, BP was 199±7 and 166±9 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01; mean arterial pressure was 185.2±6.6 and 150.5±4.1 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01). The expression of α-CGRP mRNA in DRG (122.87±3.67 arbitrary units, p<0.05), the level of CGRP in the plasma (75.40±4.99 pg/ml, p<0.01) and the density of CGRP-ir fibers in mesenteric artery (525.67±31.42 intersections, p<0.05) were significantly increased in 2K1C rats. Treatment with capsaicin, a single injection or a second injection, prevented the increased in the expression of CGRP mRNA in DRG. However, the decreased level of CGRP was only observed in the rats treated with a second capsaicin. These results suggest that in 2K1C hypertensive rats, the activity of capsaicin-sensitive sensory nerves is increased, which is playing a compensatory depressor role to partially counteract the increase in blood pressure, and that the cardiovascular actions of CGRP is mediated by the α-CGRP isoform.
AB - Previous investigations have indicated that capsaicin-sensitive sensory nerves play an important role in modulation of the peripheral resistance of the circulation system. In the present study, we examined the role of capsaicin-sensitive sensory nerves in two-kidney, one-clip (2K1C) renovascular hypertension rats. Systolic blood pressure (BP) was monitored by the tail-cuff method throughout the experiment. Concentrations of calcitonin gene-related peptide (CGRP) in the plasma, the level of CGRP mRNA in dorsal root ganglia (DRG) and the density of CGRP immunoreactive (CGRP-ir) fibers in mesenteric artery were measured. Blood pressure was significantly elevated at day 10 postoperation (BP was 143±10 and 114±7 mm Hg for 2K1C and Sham groups, respectively, p<0.05). Treatment with capsaicin, which selectively depletes neurotransmitters in sensory nerves, enhanced hypertensive responses to clipping (BP was 168±7 and 143±10 mm Hg at day 10 postoperation for Cap1+2K1C and 2K1C groups, respectively, p<0.05), and BP in the rats treated with a second injection of capsaicin was greater than that in the rats treated with a single injection of capsaicin (At day 30 postoperation, BP was 199±7 and 166±9 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01; mean arterial pressure was 185.2±6.6 and 150.5±4.1 mm Hg for Cap2+2K1C and 2K1C groups, respectively, p<0.01). The expression of α-CGRP mRNA in DRG (122.87±3.67 arbitrary units, p<0.05), the level of CGRP in the plasma (75.40±4.99 pg/ml, p<0.01) and the density of CGRP-ir fibers in mesenteric artery (525.67±31.42 intersections, p<0.05) were significantly increased in 2K1C rats. Treatment with capsaicin, a single injection or a second injection, prevented the increased in the expression of CGRP mRNA in DRG. However, the decreased level of CGRP was only observed in the rats treated with a second capsaicin. These results suggest that in 2K1C hypertensive rats, the activity of capsaicin-sensitive sensory nerves is increased, which is playing a compensatory depressor role to partially counteract the increase in blood pressure, and that the cardiovascular actions of CGRP is mediated by the α-CGRP isoform.
KW - Blood pressure
KW - Calcitonin gene-related peptide (CGRP)
KW - Capsaicin
KW - Dorsal root ganglion (DRG)
KW - Mesenteric artery
KW - Renovascular hypertension
UR - http://www.scopus.com/inward/record.url?scp=0038798656&partnerID=8YFLogxK
U2 - 10.1016/S0167-0115(03)00124-1
DO - 10.1016/S0167-0115(03)00124-1
M3 - Article
C2 - 12832107
AN - SCOPUS:0038798656
SN - 0167-0115
VL - 114
SP - 175
EP - 182
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 2-3
ER -