An in vivo model of double-unit cord blood transplantation that correlates with clinical engraftment

Lamis K. Eldjerou, Sonali Chaudhury, Ada Baisre-de Leon, Mai He, Maria E. Arcila, Glenn Heller, Richard J. O'Reilly, Juliet N. Barker, Malcolm A. Moore

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Double-unit cord blood transplantation (DCBT) appears to enhance engraftment despite sustained hematopoiesis usually being derived from a single unit. To investigate DCBT biology, in vitro and murine models were established using cells from 39 patient grafts. Mononuclear cells (MNCs) and CD34 + cells from each unit alone and in DCB combination were assessed for colony-forming cell and cobblestone area-forming cell potential, and multilineage engraftment in NOD/SCID/IL2R-γnull mice. In DCB assays, the contribution of each unit was measured by quantitative short tandem repeat region analysis. There was no correlation between colony-forming cell (n = 10) or cobblestone area-forming cell (n = 9) numbers and clinical engraftment, and both units contributed to DCB cocultures. In MNC transplantations in NOD/SCID/IL2R-γnull mice, each unit engrafted alone, but MNC DCBT demonstrated single-unit dominance that correlated with clinical engraftment in 18 of 21 cases (86%, P < .001). In contrast, unit dominance and clinical correlation were lost with CD34+ DCBT (n = 11). However, addback of CD34- to CD34+ cells (n = 20) restored single-unit dominance with the dominant unit correlating not with clinical engraftment but also with the origin of the CD34- cells in all experiments. Thus, unit dominance is an in vivo phenomenon probably associated with a graft-versus-graft immune interaction mediated by CD34- cells.

Original languageEnglish
Pages (from-to)3999-4006
Number of pages8
Issue number19
StatePublished - Nov 11 2010


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