An in vivo analysis of Miromesh - A novel porcine liver prosthetic created by perfusion decellularization

Clayton C. Petro, Ajita S. Prabhu, Lijia Liu, Arnab Majumder, James M. Anderson, Michael J. Rosen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Background Bioprosthetics derived from human or porcine dermis and intestinal submucosa have dense, homogenous, aporous collagen structures that potentially limit cellular penetration, undermining the theoretical benefit of a "natural" collagen scaffold. We hypothesized that Miromesh - a novel prosthetic derived from porcine liver by perfusion decellularization - provides a more optimal matrix for tissue ingrowth. Methods Thirty rats underwent survival surgery that constituted the creation of a 4 × 1 cm abdominal defect and simultaneous bridged repair. Twenty rats were bridged with Miromesh, and 10 rats were bridged with non-cross-linked porcine dermis (Strattice). Ten Miromesh and all 10 Strattice were rinsed in vancomycin solution and inoculated with 104 colony-forming units of green fluorescent protein-labeled Staphylococcus aureus (GFP-SA) after implantation. Ten Miromesh controls were neither soaked nor inoculated. No animals received systemic antibiotics. All animals were euthanized at 90 d and underwent an examination of their gross appearance before being sectioned for quantitative bacterial culture and histologic grading. A pathologist scored specimens (0-4) for cellular infiltration, acute inflammation, chronic inflammation, granulation tissue, foreign body reaction, and fibrous capsule formation. Results All but one rat repaired with Strattice survived until the 90-d euthanization. All quantitative bacterial cultures for inoculated specimens were negative for GFP-SA. Of nine Strattice explants, none received a cellular infiltration score >0, consistent with a poor tissue-mesh interface observed grossly. Of 10 Miromesh explants also inoculated with GFP-SA, seven of 10 demonstrated cellular infiltration with an average score of +2.7 ± 0.8, whereas sterile Miromesh implants received an average score of 0.8 ± 1.0. Two inoculated Miromesh implants demonstrated acute inflammation and infection on histology. Conclusions A prosthetic generated from porcine liver by perfusion decellularization provides a matrix for superior cellular infiltration compared with non-cross-linked porcine dermis.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalJournal of Surgical Research
Issue number1
StatePublished - Mar 1 2016


  • Biologic
  • Bioprosthetic
  • Cellular infiltration
  • Contamination
  • Hernia
  • Incisional
  • Mesh
  • Perfusion decellularization
  • Ventral


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