An In Vitro Brain Endothelial Model for Studies of Cryptococcal Transmigration into the Central Nervous System

Felipe H. Santiago-Tirado, Robyn S. Klein, Tamara L. Doering

Research output: Contribution to journalArticlepeer-review

Abstract

Cryptococcus neoformans is an environmental yeast found worldwide that causes lethal brain infections, particularly in immunocompromised hosts. In 2016, there were 280,000 cases of cryptococcal meningitis in the HIV+ population, two-thirds of them fatal; other immunocompromised patients are also affected. The burden of cryptococcal disease and the limits of current chemotherapy create a pressing need for improved treatment. One hindrance to the development of new therapies is lack of understanding of how this pathogen breaches the barriers protecting the brain. Here we describe a tool for investigating this process. This simple in vitro blood-brain-barrier (BBB) model, based on a human brain endothelial cell line grown on a permeable membrane, may be used to assay the BBB transmigration of C. neoformans or other neurotropic pathogens.

Original languageEnglish
Article numbere78
JournalCurrent Protocols in Microbiology
Volume53
Issue number1
DOIs
StatePublished - Jun 2019

Keywords

  • Cryptococcus neoformans
  • blood–brain barrier
  • cryptococcosis
  • fungal meningitis
  • hCMEC/D3
  • transwell permeable supports

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