An improved reverse transcription-polymerase chain reaction method to study apolipoprotein gene expression in Caco-2 cells

F. Giannoni, F. J. Field, N. O. Davidson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

We report a method for the detection and analysis of apolipoprotein B mRNA using the thermostable enzyme rTth to perform coupled reverse transcription- polymerase chain reaction (RT-PCR) amplification. This method, which is at least a 100-fold more sensitive than traditional RT-PCR, was used to examine elements of apolipoprotein B (apoB) gene expression in Caco-2 cells. A region of apoB mRNA spanning the edited site was amplified from pre- and postconfluent Caco-2 cells both under different growth conditions and following alterations in exogenous lipid flux to determine changes in posttranscriptional editing. Apolipoprotein A-IV (apoA-IV) mRNA levels were examined in the same samples. The results suggest that apoB mRNA editing increases in Caco-2 cells during growth but this response is more variable than previously reported. Additionally, evidence was found for differential editing of the 14 kb and 7 kb transcripts. By contrast, there was a consistent growth-related increase in apoA-IV mRNA abundance. Neither apoB mRNA editing nor apoA-IV mRNA abundance was modulated in postconfluent cells in response to different combinations of exogenous lipid. This method should facilitate the study of apolipoprotein gene expression in Caco-2 cells and other situations where the target RNA is limited either as a result of low abundance or limiting tissue sample size.

Original languageEnglish
Pages (from-to)340-350
Number of pages11
JournalJournal of lipid research
Volume35
Issue number2
StatePublished - 1994

Keywords

  • cellular differentiation
  • mRNA abundance

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