@article{e9217aefed4d484c81d9551a2182ab9e,
title = "An improved method for the retrieval of cultured macrophages",
abstract = "We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on dialysis membrane supports. Culture units are easily constructed from plastic petri dishes and dialysis membranes. Macrophages adhere readily but are detached easily from the dialysis membrane by mild mechanical agitation. Cell viability remains high and the morphologic and functional characteristics of the macrophages are maintained.",
keywords = "cell retrieval, dialysis membranes, macrophage cell culture",
author = "Fallon, \{Michael D.\} and Kahn, \{Arnold J.\} and David Ellerbrake and Teitelbaum, \{Steven L.\}",
note = "Funding Information: Cultured cells may be removed from their supporting substrate by a variety of techniques, such as: (1) exposure to cold, EDTA or lidocaine; (2) enzymatic digestion; or (3) mechanical agitation (Pennline, 1981). These methods, however, may be incomplete or result in significant cell damage. Macrophage cultures, for example, adhere tenaciously to polystyrene or glass petri dishes and are difficult to remove by the usual techniques. Trypsin digestion, on the other hand, may alter cell surface morphology and membrane receptor status. We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on cellulose dialysis membrane supports. These cells adhere readily, but are detached easily from the dialysis membrane by mild agitation. Cell viability remains high and morphologic and functional characteristics of macrophages are maintained. Specifically, the Supported in part by NIH Training Grant no. AM07033 and NIH Grant nos. DE05413 and AM06756. 2 Address reprint requests to: Michael D. Fallon, M.D., Department of Pathology and Laboratory Medicine, The Jewish Hospital of St. Louis, 216 South Kingshighway, St. Louis, MO 63110, U.S.A. 3 Deceased.",
year = "1982",
month = nov,
day = "12",
doi = "10.1016/0022-1759(82)90322-2",
language = "English",
volume = "54",
pages = "379--384",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
number = "3",
}