An improved method for the retrieval of cultured macrophages

Michael D. Fallon, Arnold J. Kahn, David Ellerbrake, Steven L. Teitelbaum

Research output: Contribution to journalArticle

Abstract

We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on dialysis membrane supports. Culture units are easily constructed from plastic petri dishes and dialysis membranes. Macrophages adhere readily but are detached easily from the dialysis membrane by mild mechanical agitation. Cell viability remains high and the morphologic and functional characteristics of the macrophages are maintained.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalJournal of Immunological Methods
Volume54
Issue number3
DOIs
StatePublished - Nov 12 1982

Keywords

  • cell retrieval
  • dialysis membranes
  • macrophage cell culture

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