An evaluation of the inactive mouse X chromosome in somatic cell hybrids

Eduardo C. Salido; Merry B. Passage; Pauline H. Yen; Larry J. Shapiro; T. K. Mohandas

doi:10.1007/BF01233955

An evaluation of the inactive mouse X chromosome in somatic cell hybrids

Eduardo C. Salido, Merry B. Passage, Pauline H. Yen, Larry J. Shapiro, T. K. Mohandas

Research output: Contribution to journal › Article

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Abstract

The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamstermouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X. These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F₁ females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.

Original language	English
Pages (from-to)	65-71
Number of pages	7
Journal	Somatic Cell and Molecular Genetics
Volume	19
Issue number	1
DOIs	https://doi.org/10.1007/BF01233955
State	Published - Jan 1 1993

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Salido, E. C., Passage, M. B., Yen, P. H., Shapiro, L. J., & Mohandas, T. K. (1993). An evaluation of the inactive mouse X chromosome in somatic cell hybrids. Somatic Cell and Molecular Genetics, 19(1), 65-71. https://doi.org/10.1007/BF01233955

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AU - Mohandas, T. K.

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