TY - JOUR
T1 - An evaluation of the inactive mouse X chromosome in somatic cell hybrids
AU - Salido, Eduardo C.
AU - Passage, Merry B.
AU - Yen, Pauline H.
AU - Shapiro, Larry J.
AU - Mohandas, T. K.
PY - 1993/1
Y1 - 1993/1
N2 - The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamstermouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X. These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F1 females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.
AB - The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamstermouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X. These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F1 females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.
UR - http://www.scopus.com/inward/record.url?scp=0027238240&partnerID=8YFLogxK
U2 - 10.1007/BF01233955
DO - 10.1007/BF01233955
M3 - Article
C2 - 8460399
AN - SCOPUS:0027238240
VL - 19
SP - 65
EP - 71
JO - Somatic Cell and Molecular Genetics
JF - Somatic Cell and Molecular Genetics
SN - 0740-7750
IS - 1
ER -