An Evaluation of Receptor‐Mediated Gene Transfer Using Synthetic DNA‐Ligand Complexes

Jose C. Perales, Thomas Ferkol, Maria Molas, Richard W. Hanson

Research output: Contribution to journalReview articlepeer-review

159 Scopus citations


Receptor‐mediated gene transfer is an attractive method for therapeutically correcting human genetic diseases since it permits the targeting of DNA to cellular receptors in specific tissues of adult animals. Genes introduced by this technique have been shown to be expressed in the target tissue for varying periods. However, to be useful for gene therapy, it is critical that both the chemical properties and physical interactions of the reagents involved in the design of the DNA delivery vehicle be rigorously characterized. In this review, we discuss the critical steps in the preparation of the DNA‐ligand complex and the factors involved in the delivery and regulated expression of a transgene in animal tissues. The feasibility of using this technique for the therapeutic delivery of genes to mammalian tissues will also be evaluated.

Original languageEnglish
Pages (from-to)255-266
Number of pages12
JournalEuropean Journal of Biochemistry
Issue number2
StatePublished - Dec 1994


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