An enzymatic method for the detection of human serum albumin

Masood Ul Hassan Javed; Saima N. Waqar

doi:10.1038/emm.2001.18

An enzymatic method for the detection of human serum albumin

Masood Ul Hassan Javed
, Saima N. Waqar

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

Original language	English
Pages (from-to)	103-105
Number of pages	3
Journal	Experimental and Molecular Medicine
Volume	33
Issue number	2
DOIs	https://doi.org/10.1038/emm.2001.18
State	Published - Jun 30 2001

Keywords

Albumin
Human
LDH-5
Serum

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10.1038/emm.2001.18

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abstract = "Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.",

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AU - Waqar, Saima N.

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N2 - Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

AB - Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

KW - Albumin

KW - Human

KW - LDH-5

KW - Serum

UR - https://www.scopus.com/pages/publications/0035973193

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JO - Experimental and Molecular Medicine

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ER -

An enzymatic method for the detection of human serum albumin

Abstract

Keywords

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