An enzymatic method for the detection of human serum albumin

Masood Ul Hassan Javed, Saima N. Waqar

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

Original languageEnglish
Pages (from-to)103-105
Number of pages3
JournalExperimental and Molecular Medicine
Volume33
Issue number2
StatePublished - Jun 30 2001
Externally publishedYes

Keywords

  • Albumin
  • Human
  • LDH-5
  • Serum

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