Abstract
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Original language | English |
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Pages (from-to) | 103-105 |
Number of pages | 3 |
Journal | Experimental and Molecular Medicine |
Volume | 33 |
Issue number | 2 |
DOIs | |
State | Published - Jun 30 2001 |
Keywords
- Albumin
- Human
- LDH-5
- Serum