An ELISA for detection of apoptosis

Padmini Salgame, Arun S. Varadhachary, Laura L. Primiano, John E. Fincke, Sylviane Muller, Marc Monestier

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.

Original languageEnglish
Pages (from-to)680-681
Number of pages2
JournalNucleic acids research
Volume25
Issue number3
DOIs
StatePublished - Jul 30 1997
Externally publishedYes

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    Salgame, P., Varadhachary, A. S., Primiano, L. L., Fincke, J. E., Muller, S., & Monestier, M. (1997). An ELISA for detection of apoptosis. Nucleic acids research, 25(3), 680-681. https://doi.org/10.1093/nar/25.3.680