An ELISA for detection of apoptosis

Padmini Salgame; Arun S. Varadhachary; Laura L. Primiano; John E. Fincke; Sylviane Muller; Marc Monestier

doi:10.1093/nar/25.3.680

An ELISA for detection of apoptosis

Padmini Salgame, Arun S. Varadhachary, Laura L. Primiano, John E. Fincke, Sylviane Muller, Marc Monestier

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Abstract

We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.

Original language	English
Pages (from-to)	680-681
Number of pages	2
Journal	Nucleic acids research
Volume	25
Issue number	3
DOIs	https://doi.org/10.1093/nar/25.3.680
State	Published - 1997

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title = "An ELISA for detection of apoptosis",

abstract = "We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.",

author = "Padmini Salgame and Varadhachary, {Arun S.} and Primiano, {Laura L.} and Fincke, {John E.} and Sylviane Muller and Marc Monestier",

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AU - Salgame, Padmini

AU - Varadhachary, Arun S.

AU - Primiano, Laura L.

AU - Fincke, John E.

AU - Muller, Sylviane

AU - Monestier, Marc

N1 - Funding Information: This work was supported by NIH Grants AI-26665 and AI-40160, and the Potts Memorial Foundation.

PY - 1997

Y1 - 1997

N2 - We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.

AB - We describe a simple and convenient enzyme-linked immunosorbent assay (ELISA) for the detection of apoptosis in tissue culture. An early event in apoptosis is DNA fragmentation followed by release of nucleosomes into the cytoplasm. Our sandwich assay uses a pair of monoclonal antibodies specific for two nucleosomal epitopes to capture and detect cytoplasmic nucleosomes onto the ELISA plate. Our assay is about 500 times more sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially suited for the testing of large numbers of samples.

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U2 - 10.1093/nar/25.3.680

DO - 10.1093/nar/25.3.680

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VL - 25

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EP - 681

JO - Nucleic acids research

JF - Nucleic acids research

IS - 3

ER -

An ELISA for detection of apoptosis

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