An azimuthal polarizer assures localization accuracy in single-molecule super-resolution fluorescence microscopy

Matthew D. Lew, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Imaging only azimuthally-polarized light from single fluorescent molecules avoids emission from the z component of their transition dipole moments, resulting in accurate measurement of location regardless of emitter orientation and degree of objective lens misfocus.

Original languageEnglish
Title of host publication2015 Conference on Lasers and Electro-Optics, CLEO 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781557529688
StatePublished - Aug 10 2015
EventConference on Lasers and Electro-Optics, CLEO 2015 - San Jose, United States
Duration: May 10 2015May 15 2015

Publication series

NameConference on Lasers and Electro-Optics Europe - Technical Digest
Volume2015-August

Conference

ConferenceConference on Lasers and Electro-Optics, CLEO 2015
Country/TerritoryUnited States
CitySan Jose
Period05/10/1505/15/15

Keywords

  • Accuracy
  • Fluorescence
  • Image resolution
  • Lenses
  • Microscopy
  • Optical microscopy

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