An Athymic Rat Model for Mandibular Osteoradionecrosis Allowing for Direct Translation of Regenerative Treatments

Ryan S. Jackson, Stephen G. Voss, Zachary C. Wilson, Nicholas B. Remmes, Paul G. Stalboerger, Michael G. Keeney, Eric J. Moore, Jeffrey R. Janus

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objective We aim to create a model of mandibular osteoradionecrosis in athymic rats. Athymic rats provide an immunosuppressed environment whereby human stem cells and biomaterials can be used to investigate regenerative solutions for osteoradionecrosis, bridging the gap between in vivo testing and clinical application. Study Design Prospective animal study. Setting Academic otolaryngology department laboratory. Subjects and Methods After Institutional Animal Care and Use Committee approval, 10 athymic nude rats were divided into 2 groups. The experimental group (n = 6) underwent irradiation (20 Gy), while the control group (n = 4) underwent sham irradiation catheter placement only. All 10 rats underwent extraction of the second mandibular molar 7 days later. The rats were sacrificed 28 days after dental extraction, and their mandibles were harvested. The mandibles were examined with histologic analysis and bone volume analysis based on 3-dimensional micro-computed tomography. Results All 10 rats survived the experiment period. Radiographic and histologic analysis revealed decreased bone formation in the experimental group compared with the control group. Jaw region volume ratio was 0.83 for the experimental group versus 0.97 in the control group (P =.003). The region-of-interest volume ratio was 0.75 in the experimental group and 0.97 in the control group (P =.005). Histologically, there were increased osteoclasts (P =.02) and decreased osteoblasts (P =.001) as well as increased fibrosis in the experimental group versus the control group. Conclusion Mandibular osteoradionecrosis can be effectively and reproducibly produced in an athymic rat model. This will allow further research to study regenerative medicine in an athymic rat model.

Original languageEnglish
Pages (from-to)526-531
Number of pages6
JournalOtolaryngology - Head and Neck Surgery (United States)
Volume153
Issue number4
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Keywords

  • animal model
  • mandible
  • osteoradionecrosis
  • translational research

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