TY - JOUR
T1 - An Approach for Improvement of Carbon Fiber Technique to Study Cardiac Cell Contractility
AU - Myachina, T.
AU - Khokhlova, A.
AU - Antsygin, I.
AU - Lookin, O.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/5/14
Y1 - 2018/5/14
N2 - The technologies to study cardiac cell mechanics in near-physiological conditions are limited. Carbon fiber (CF) technique is a unique tool to study single cardiomyocyte contractility. However, the CF adhesion to a cell is limited and it is difficult to control CF sliding occurred due to inappropriate adhesion. In this study, we present a CF adhesion quality index - a linear coefficient (slope) derived from "end-diastolic cell length - end-diastolic sarcomere length" relationship. Potential applicability of this index is demonstrated on isolated rat and guinea pig ventricular cardiomyocytes. Further improvement of the approach may help to increase the quality of the experimental data obtained by CF technique.
AB - The technologies to study cardiac cell mechanics in near-physiological conditions are limited. Carbon fiber (CF) technique is a unique tool to study single cardiomyocyte contractility. However, the CF adhesion to a cell is limited and it is difficult to control CF sliding occurred due to inappropriate adhesion. In this study, we present a CF adhesion quality index - a linear coefficient (slope) derived from "end-diastolic cell length - end-diastolic sarcomere length" relationship. Potential applicability of this index is demonstrated on isolated rat and guinea pig ventricular cardiomyocytes. Further improvement of the approach may help to increase the quality of the experimental data obtained by CF technique.
UR - https://www.scopus.com/pages/publications/85050686880
U2 - 10.1088/1757-899X/350/1/012011
DO - 10.1088/1757-899X/350/1/012011
M3 - Conference article
AN - SCOPUS:85050686880
SN - 1757-8981
VL - 350
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012011
T2 - 2017 International Conference on Nanomaterials and Biomaterials, ICNB 2017
Y2 - 11 December 2017 through 13 December 2017
ER -